Second near-infrared fluorescent dye for lateral flow immunoassays rapid detection of influenza A/B virus

Runxuan Zhang; Tao Liao; Xiao Wang; Hong Zhai; Di Yang; Xin Wang; Haiyan Wang; Feng Feng

doi:10.1016/j.ab.2022.114847

Second near-infrared fluorescent dye for lateral flow immunoassays rapid detection of influenza A/B virus

Anal Biochem. 2022 Oct 15:655:114847. doi: 10.1016/j.ab.2022.114847. Epub 2022 Aug 12.

Authors

Runxuan Zhang¹, Tao Liao², Xiao Wang³, Hong Zhai¹, Di Yang¹, Xin Wang¹, Haiyan Wang⁴, Feng Feng¹

Affiliations

¹ Department of Chemistry and Chemical Engineering, Shanxi Provincial Key Laboratory of Chemical Biosensing, China, Shanxi Datong University, Datong, 037009, China.
² WWHS Biotech, Inc, China, Shenzhen, 518000, China.
³ Institute of Public Security, Northwest University of Political Science and Law, China, Xi'an, 710122, China.
⁴ Department of Chemistry and Chemical Engineering, Shanxi Provincial Key Laboratory of Chemical Biosensing, China, Shanxi Datong University, Datong, 037009, China. Electronic address: why7135280@126.com.

PMID: 35964731
DOI: 10.1016/j.ab.2022.114847

Abstract

Sensitive and rapid diagnostic point of care testing (POCT) system is of great significance to prevent and control human virus infection. Here reported an immunochromatographic strip technology. The second near-infrared (NIR-II) fluorescent dye encapsulated into polystyrene (PS) nanoparticles, was integrated into a lateral flow assay platform to achieve excellent detection of influenza A/B. This surface-functionalized and mono-dispersed PS nanoparticles has been conjugated with influenza nucleoprotein monoclonal antibody as targets for influenza antigen-detection. This assay achieved the detection limit of 0.015 ng/mL for influenza A nucleoprotein and 4.3*10^-5 HAU/mL (10^2.08 TCID₅₀/mL) influenza A virus (influenza B: 0.037 ng/mL, 9.7*10^-7 HAU/mL (10^0.43 TCID₅₀/mL)). Compared with an Au-based lateral flow test strip, the strip's sensitivity is about 16-fold higher than it. Strip detection properties remain stable for 6 months under 4 °C to 30 °C storage. The assay's intra assay variation is 5.14% and the inter assay variation is 7.74%. Other potential endogenous and exogenous interfering substances (whole blood, nasal mucin, saliva, antipyretics, antihistamines and neuraminidase inhibitors) showed negative results, which verified the excellent specificity of this method. This assay was successfully applied to the POCT quantitative detection of influenza A/B virus, the sensitivity to influenza A and B viruses was 70% and 87.5% respectively, and the specificity was 100%. Therefore, these microspheres can be used as an effective material for rapid POCT detection in clinical specimens.

Keywords: Influenza A/B virus; Lateral flow immunoassay; Second near-infrared.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Viral
Fluorescent Dyes
Herpesvirus 1, Cercopithecine*
Humans
Immunoassay / methods
Influenza A virus*
Influenza B virus
Influenza, Human* / diagnosis
Nucleoproteins
Sensitivity and Specificity

Substances

Antibodies, Viral
Fluorescent Dyes
Nucleoproteins