The microRNAs in the antennae of Apolygus lucorum (Hemiptera: Miridae): Expression properties and targets prediction

Adel Khashaveh; Xingkui An; Shuang Shan; Xiaoqian Pang; Yan Li; Xiaowei Fu; Yongjun Zhang

doi:10.1016/j.ygeno.2022.110447

The microRNAs in the antennae of Apolygus lucorum (Hemiptera: Miridae): Expression properties and targets prediction

Genomics. 2022 Sep;114(5):110447. doi: 10.1016/j.ygeno.2022.110447. Epub 2022 Aug 11.

Authors

Adel Khashaveh¹, Xingkui An¹, Shuang Shan¹, Xiaoqian Pang², Yan Li³, Xiaowei Fu⁴, Yongjun Zhang⁵

Affiliations

¹ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
² State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; School of Resource and Environment, Henan Institute of Science and Technology, Xinxiang 453003, China.
³ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, College of Life Sciences, Anhui Normal University, Wuhu, Anhui 241000, China.
⁴ School of Resource and Environment, Henan Institute of Science and Technology, Xinxiang 453003, China.
⁵ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: yjzhang@ippcaas.cn.

PMID: 35963492
DOI: 10.1016/j.ygeno.2022.110447

Abstract

MicroRNAs (miRNAs) regulate gene expression and contribute to numerous physiological processes. However, little is known about the functions of miRNAs in insect chemosensation. In this study, nine small RNA libraries were constructed and sequenced from the antennae of nymphs, adult males, and adult females of Apolygus lucorum. In total, 399 (275 known and 124 novel) miRNAs were identified. miR-7-5p_1 was the most abundant miRNA. Altogether, 69,708 target genes related to biogenesis, membrane, and binding activities were predicted. In particular, 15 miRNAs targeted 16 olfactory genes. Comparing the antennae of nymphs and adult males and females, 94 miRNAs were differentially expressed. Alternatively, a subset of differentially expressed miRNAs was verified by qPCR, supporting the reliability of the sequencing results. This study provides a global miRNA transcriptome for the antennae of A. lucorum and valuable information for further investigations of the functions of miRNAs in the regulation of chemosensation.

Keywords: Apolygus lucorum; Chemosensory-related genes; Small RNA sequencing; Target prediction; miRNAs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Female
Heteroptera* / genetics
Male
MicroRNAs* / genetics
Nymph / genetics
Reproducibility of Results
Transcriptome

Substances

MicroRNAs