Cas12a-based on-site, rapid detection of genetically modified crops

Zhiqiang Duan; Xiaoliang Yang; Xingkun Ji; Ying Chen; Xiaomu Niu; Anping Guo; Jian-Kang Zhu; Feng Li; Zhaobo Lang; Hui Zhao

doi:10.1111/jipb.13342

Cas12a-based on-site, rapid detection of genetically modified crops

J Integr Plant Biol. 2022 Oct;64(10):1856-1859. doi: 10.1111/jipb.13342. Epub 2022 Oct 6.

Authors

Zhiqiang Duan¹, Xiaoliang Yang², Xingkun Ji¹, Ying Chen³, Xiaomu Niu³, Anping Guo², Jian-Kang Zhu^{4

5

6}, Feng Li³, Zhaobo Lang⁷, Hui Zhao²

Affiliations

¹ Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Institute of Tropical Bioscience and Biotechnology & San Ya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China.
² Bellagen Biotechnology Co. Ltd., Jinan, 250000, China.
³ College of Life Sciences, Shandong Normal University, Jinan, 250014, China.
⁴ Institute of Advanced Biotechnology and School of Life Sciences, Southern University of Science and Technology, Shenzhen, 518055, China.
⁵ Center for Advanced Bioindustry Technologies, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
⁶ Hainan Yazhou Bay Seed Lab, Sanya, Hainan, 572024, China.
⁷ Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, 201602, China.

PMID: 35962717
DOI: 10.1111/jipb.13342

Abstract

A CRISPR/LbCas12a-based nucleic acid detection method that uses crude leaf extracts as samples and is rapid (≤40 min for a full run) and highly sensitive (0.01%) can be used to monitor genetically modified organisms in the field.

MeSH terms

CRISPR-Cas Systems* / genetics
Crops, Agricultural / genetics
Nucleic Acid Amplification Techniques / methods
Nucleic Acids*
Plant Extracts
Plants, Genetically Modified / genetics

Substances

Nucleic Acids
Plant Extracts