A novel biomimetic network amplification strategy designed fluorescent aptasensor based on yolk-shell Fe3O4 nanomaterials for aflatoxin B1 detection

Xiaoze Dong; Shuo Qi; Mingwei Qin; Yuhan Sun; Yan Lv; Yin Zhang; Zhouping Wang

doi:10.1016/j.foodchem.2022.133761

A novel biomimetic network amplification strategy designed fluorescent aptasensor based on yolk-shell Fe₃O₄ nanomaterials for aflatoxin B1 detection

Food Chem. 2023 Jan 1:398:133761. doi: 10.1016/j.foodchem.2022.133761. Epub 2022 Jul 22.

Authors

Xiaoze Dong¹, Shuo Qi¹, Mingwei Qin¹, Yuhan Sun¹, Yan Lv¹, Yin Zhang², Zhouping Wang³

Affiliations

¹ State Key Laboratory of Food Science and Technology, International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China.
² Key Laboratory of Meat Processing of Sichuan, Chengdu University, Chengdu 610106, China.
³ State Key Laboratory of Food Science and Technology, International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Meat Processing of Sichuan, Chengdu University, Chengdu 610106, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Food, Jiangnan University, Wuxi 214122, China. Electronic address: wangzp@jiangnan.edu.cn.

PMID: 35961167
DOI: 10.1016/j.foodchem.2022.133761

Abstract

In this study, yolk-shell Fe₃O₄-based nanomaterials designing of fluorescent aptasensor using a new type of biomimetic network-constitutional dynamic networks (CDNs) amplification strategy was developed for aflatoxin B1 (AFB1) detection. By ingeniously designing the base sequences A, a, B, b, and coupling with endonuclease (BbvCI), a constitutes of CDNs is formed as a fluorescence intensity amplifier. With such design, the as- prepared aptasensor exhibits good sensitivity from 50 fg·mL^-1-50 ng·mL^-1 with a detection limit of 35.94 fg·mL^-1. Moreover, the CDNs can achieve 1000-fold amplification in terms of linear range and detection limit. The results confirmed that this new type of biomimetic network amplification strategy can supply-one efficient approach to improve signal amplification. Furthermore, the prepared aptamer sensor was tested for spiked recovery in peanut samples, and the recoveries ranged from 83.79 to 95.06 %, which has confirmed its practical application value in the field of food safety.

Keywords: AFB(1); Aptamer; Constitutional dynamic networks; Fe(3)O(4); Yolk-shell.

MeSH terms

Aflatoxin B1 / analysis
Aptamers, Nucleotide*
Biomimetics
Biosensing Techniques* / methods
Limit of Detection
Metal Nanoparticles*
Nanostructures*

Substances

Aptamers, Nucleotide
Aflatoxin B1