Recent progress in dissecting ubiquitin signals with chemical biology tools

Qingyun Zheng; Zhen Su; Yuanyuan Yu; Lei Liu

doi:10.1016/j.cbpa.2022.102187

Recent progress in dissecting ubiquitin signals with chemical biology tools

Curr Opin Chem Biol. 2022 Oct:70:102187. doi: 10.1016/j.cbpa.2022.102187. Epub 2022 Aug 9.

Authors

Qingyun Zheng¹, Zhen Su², Yuanyuan Yu³, Lei Liu⁴

Affiliations

¹ School of Medicine, Shanghai Engineering Research Center of Organ Repair, Shanghai University, Shanghai 200444, China; Tsinghua-Peking Center for Life Sciences, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (MOE), Department of Chemistry, Tsinghua University, Beijing, 100084, China.
² School of Medicine, Shanghai Engineering Research Center of Organ Repair, Shanghai University, Shanghai 200444, China.
³ School of Medicine, Shanghai Engineering Research Center of Organ Repair, Shanghai University, Shanghai 200444, China. Electronic address: yuyy@shu.edu.cn.
⁴ Tsinghua-Peking Center for Life Sciences, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (MOE), Department of Chemistry, Tsinghua University, Beijing, 100084, China. Electronic address: lliu@mail.tsinghua.edu.cn.

PMID: 35961065
DOI: 10.1016/j.cbpa.2022.102187

Abstract

Protein ubiquitination regulates almost all eukaryotic cellular processes, and is of very high complexity due to the diversity of ubiquitin (Ub) modifications including mono-, multiply mono-, homotypic poly-, and even heterotypic poly-ubiquitination. To accurately elucidate the role of each specific Ub signal in different cells with spatiotemporal resolutions, a variety of chemical biology tools have been developed. In this review, we summarize some recently developed chemical biology tools for ubiquitination studies and their applications in molecular and cellular biology.

Keywords: Free Ub sensor; Linkage-specific Ub chain detection reagent; Ub antitrypsin protector; Ub chain probe; Ub clipping tool.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Biology*
Ubiquitin* / chemistry
Ubiquitination

Substances

Ubiquitin