Heat Shock Proteins 70 Regulate Cell Motility and Invadopodia-Associated Proteins Expression in Oral Squamous Cell Carcinoma

Le-Xi Ding; Jing Zhang; Si-Si Yang; Jin Wu; Tong Su; Wei-Ming Wang

doi:10.3389/fendo.2022.890218

Heat Shock Proteins 70 Regulate Cell Motility and Invadopodia-Associated Proteins Expression in Oral Squamous Cell Carcinoma

Front Endocrinol (Lausanne). 2022 Jul 26:13:890218. doi: 10.3389/fendo.2022.890218. eCollection 2022.

Authors

Le-Xi Ding^{1

2

3}, Jing Zhang^{4

5}, Si-Si Yang^{4

6}, Jin Wu^{4

6}, Tong Su⁴, Wei-Ming Wang⁴

Affiliations

¹ Eye Center of Xiangya Hospital, Central South University, Changsha, China.
² National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Changsha, China.
³ Hunan Key Laboratory of Ophthalmology, Central South University, Changsha, China.
⁴ Department of Oral and Maxillofacial Surgery, Center of Stomatology, Xiangya Hospital, Central South University, Changsha, China.
⁵ Institute of Oral Precancerous Lesions, Central South University, Changsha, China.
⁶ Research Center of Oral and Maxillofacial Tumor, Xiangya Hospital, Central South University, Changsha, China.

Abstract

Background: Many studies have shown that diabetes is often closely related to oral squamous cell carcinoma (OSCC) occurrence and metastasis. Heat shock protein 70 (Hsp70) is a molecular chaperone related to diabetes complications. This study aims to investigate the role of Hsp70 in OSCC in expression of invadopodia-associated proteins.

Methods: The expressions and correlation of HSP70, Hif1α, MMP2, MMP14, and cortactin were examined using bioinformatics analysis and verified by OSCC tissue microarrays. Assay in vitro was performed to analyze cell migration capacity after treatment with or without the HSP70 inhibitor.

Results: The expressions of invadopodia-associated proteins were enhanced in OSCC tissues compared with paracarcinoma tissues and partially correlated with HSP70. Inhibiting HSP70 significantly decreased the cell viability, proliferation, and migration of OSCC cells.

Conclusions: HSP70 may be involved in invadopodia-associated proteins in OSCC cells, which provides a promising method for treatment of OSCC metastasis.

Keywords: Hif1α; bioinformatics analysis; heat shock proteins 70; invadopodia-associated proteins; oral squamous cell carcinoma.

MeSH terms

Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / metabolism
Carcinoma, Squamous Cell / pathology
Cell Movement / genetics
Cell Movement / physiology
HSP70 Heat-Shock Proteins* / genetics
HSP70 Heat-Shock Proteins* / metabolism
Head and Neck Neoplasms* / genetics
Head and Neck Neoplasms* / metabolism
Head and Neck Neoplasms* / pathology
Humans
Mouth Neoplasms* / genetics
Mouth Neoplasms* / metabolism
Mouth Neoplasms* / pathology
Neoplasm Metastasis / genetics
Neoplasm Metastasis / physiopathology
Podosomes* / metabolism
Podosomes* / pathology
Squamous Cell Carcinoma of Head and Neck* / genetics
Squamous Cell Carcinoma of Head and Neck* / metabolism
Squamous Cell Carcinoma of Head and Neck* / pathology

Substances

HSP70 Heat-Shock Proteins