Recombinant Spidroin Microgel as the Base of Cell-Engineered Constructs Mediates Liver Regeneration in Rats

Murat Shagidulin; Nina Onishchenko; Anastasiia Grechina; Alla Nikolskaya; Mikhail Krasheninnikov; Aleksey Lyundup; Elena Volkova; Natalia Mogeiko; Artem Venediktov; Gennadii Piavchenko; Lubov Davydova; Alla Ramonova; Vladimir Bogush; Sergey Gautier

doi:10.3390/polym14153179

Recombinant Spidroin Microgel as the Base of Cell-Engineered Constructs Mediates Liver Regeneration in Rats

Polymers (Basel). 2022 Aug 4;14(15):3179. doi: 10.3390/polym14153179.

Authors

Affiliations

¹ Federal State Budgetary Institution "Shumakov National Medical Research Center of Transplantology and Artificial Organs" of the Ministry of Health of the Russian Federation, 123182 Moscow, Russia.
² Federal State Autonomous Educational Institution of Higher Education, "I.M. Sechenov First Moscow State Medical University" of the Ministry of Health of the Russian Federation (Sechenov University), 119435 Moscow, Russia.
³ Research and Education Resourse Center, Peoples Friendship University of Russia (RUDN University), 117198 Moscow, Russia.
⁴ National Research Center "Kurchatov Institute", 123182 Moscow, Russia.
⁵ Bioengineering Department, Faculty of Biology, M.V. Lomonosov Moscow State University, 119234 Moscow, Russia.

Abstract

Aim: In this study, we seek to check if recombinant spidroin rS1/9 is applicable for cell-engineering construct development. Novel technologies of cell and tissue engineering are relevant for chronic liver failure management. Liver regeneration may represent one of the possible treatment options if a cell-engineered construct (CEC) is used. Nowadays, one can see the continuous study of various matrices to create an appropriate CEC. Materials and Methods: We have adhered allogenic liver cells and multipotent mesenchymal bone marrow stem cells (MMSC BM) to a microgel with recombinant spidroin rS1/9. Then we have studied the developed implantable CEC in a rat model (n = 80) of chronic liver failure achieved by prolonged poisoning with carbon tetrachloride. Results: Our results demonstrate that the CECs change the values of biochemical tests and morphological parameters in chronic liver failure in rats. Conclusion: We consider there to be a positive effect from the microgel-based CECs with recombinant spidroin rS1/9 in the treatment of chronic liver failure.

Keywords: cell-engineered constructs; chronic liver failure; liver cells; matrix; mmsc bm.

Grants and funding

Experimental approaches to the creation of tissue equivalents of the liver from the Federal State Budgetary Institution “Shumakov National Medical Research Center of Transplantology and Artificial Organs” of the Ministry of Health of the Russian Federation No. 14.604.21.0001. project ID:RFMEFI60414X0001. The section of the recombinant spidroin biosynthesisand purification, hydrogel and microgel samples preparation was supported by an internal grant from the National Research Center “Kurchatov Institute” No. 2755 dated 28.10.2021. G.P. acknowledge funding from the Academy of Finland (project 351068) “Smart Standalone Digital Histopathological Tissue Characterization with Polarized Light (Smart-Light)”.