Polyphenolic Contents, Free Radical Scavenging and Cholinesterase Inhibitory Activities of Dalbergiella welwitschii Leaf Extracts

Tabisa Diniso; Jerry Adeyemi; Ayodeji Oriola; Taiwo Elufioye; Mavuto Gondwe; Adebola Oyedeji

doi:10.3390/plants11152066

Polyphenolic Contents, Free Radical Scavenging and Cholinesterase Inhibitory Activities of Dalbergiella welwitschii Leaf Extracts

Plants (Basel). 2022 Aug 8;11(15):2066. doi: 10.3390/plants11152066.

Authors

Tabisa Diniso¹, Jerry Adeyemi¹, Ayodeji Oriola¹, Taiwo Elufioye¹, Mavuto Gondwe², Adebola Oyedeji¹

Affiliations

¹ Department of Chemical and Physical Sciences, Walter Sisulu University, Mthatha 5099, South Africa.
² Department of Human Biology, Faculty of Health Sciences, Walter Sisulu University, Mthatha 5099, South Africa.

Abstract

A decoction of Dalbergiella welwitschii leaves has been used ethnomedicinally for the treatment of mental illness and inflammatory diseases amongst other diseases. In this study, the leaf methanol extract of D. welwitschii and its partition fractions: n-hexane, ethyl acetate and aqueous, were tested and evaluated for their polyphenolic contents, free radical scavenging and cholinesterase inhibitory activities. The total phenolic (TPC), flavonoid (TFC) and proanthocyanidin (TPA) contents were determined using standard colorimetric methods. The anti-radical activity of the extracts against the 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric ion and nitric oxide (NO) radicals as well as their effects on lipid peroxidation were monitored spectrophotometrically. The cholinesterase enzyme (AChE and BuChE) inhibitions by the extracts were determined by a modified method of Ellman. The result showed a concentration-dependent increase in inhibition of the free radicals and the cholinesterase enzymes, except for that of lipid peroxidation. The ethyl acetate (EtOAc) fraction exhibited the highest polyphenolic contents among the fractions, with a TPC of 1.08 mgGAE/g, TFC of 0.38 mgQuE/g and TPA of 0.21 mgGAE/g. It also demonstrated the highest free radical scavenging activities with 72.63% and 65.43% inhibitions of DPPH and NO, respectively. The EtOAc fraction inhibited AChE and BuChE enzymes with IC₅₀ values of 0.94 and 8.49 mg/mL, respectively. Our findings show that the plant may have polyphenol contents, in particular in the methanol extract and EtOAc fraction. These extracts showed considerable free radical scavenging and cholinesterase inhibitory properties. Thus, the observed bioactivities may serve as a justification for its folkloric use as a remedy for mental illness. The study also provides relevant information that could help in the search for lead cholinesterase inhibitors from medicinal plants that can be exploited against neurodegenerative disorders.

Keywords: Dalbergiella welwitschii; cholinesterase inhibitors; free radical scavengers; neurodegenerative disorders; polyphenolic contents.

Grants and funding

The authors acknowledge the National Research Foundation, South Africa (with reference number: PDG200329510773 and grant number: 129625), and Walter Sisulu University (WSU), South Africa, for funding and facilities of this project.