Static Magnetic Fields Regulate T-Type Calcium Ion Channels and Mediate Mesenchymal Stem Cells Proliferation

Haokaifeng Wu; Chuang Li; Muqaddas Masood; Zhen Zhang; Esther González-Almela; Alvaro Castells-Garcia; Gaoyang Zou; Xiaoduo Xu; Luqin Wang; Guoqing Zhao; Shengyong Yu; Ping Zhu; Bo Wang; Dajiang Qin; Jing Liu

doi:10.3390/cells11152460

Static Magnetic Fields Regulate T-Type Calcium Ion Channels and Mediate Mesenchymal Stem Cells Proliferation

Cells. 2022 Aug 8;11(15):2460. doi: 10.3390/cells11152460.

Authors

Haokaifeng Wu^{1

2}, Chuang Li³, Muqaddas Masood⁴, Zhen Zhang^{1

2}, Esther González-Almela⁵, Alvaro Castells-Garcia⁵, Gaoyang Zou⁵, Xiaoduo Xu³, Luqin Wang^{1

2}, Guoqing Zhao⁵, Shengyong Yu^{1

2}, Ping Zhu⁴, Bo Wang⁴, Dajiang Qin⁶, Jing Liu^{1

2

5}

Affiliations

¹ CAS Key Laboratory of Regenerative Biology, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
² Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
³ School of Life Sciences, University of Science and Technology of China, Hefei 230026, China.
⁴ Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510100, China.
⁵ Bioland Laboratory, Guangzhou 510005, China.
⁶ Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou 510000, China.

Abstract

The static magnetic fields (SMFs) impact on biological systems, induce a variety of biological responses, and have been applied to the clinical treatment of diseases. However, the underlying mechanisms remain largely unclear. In this report, by using human mesenchymal stem cells (MSCs) as a model, we investigated the biological effect of SMFs at a molecular and cellular level. We showed that SMF exposure promotes MSC proliferation and activates the expression of transcriptional factors such as FOS (Fos Proto-Oncogene, AP-1 Transcription Factor Subunit) and EGR1 (Early Growth Response 1). In addition, the expression of signal-transduction proteins p-ERK1/2 and p-JNK oscillate periodically with SMF exposure time. Furthermore, we found that the inhibition of the T-type calcium ion channels negates the biological effects of SMFs on MSCs. Together, we revealed that the SMFs regulate T-type calcium ion channels and mediate MSC proliferation via the MAPK signaling pathways.

Keywords: MAPK signaling pathway; T-type calcium ion channel; mesenchymal stem cell; static magnetic field.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Calcium Channels, T-Type* / metabolism
Cell Proliferation
Humans
MAP Kinase Signaling System
Magnetic Fields
Mesenchymal Stem Cells* / metabolism

Substances

Calcium Channels, T-Type

Grants and funding

This research was funded in part by National Natural Science Foundation of China (U20A2013, 32022019, 81974019, 31830060, 31530038, 31770889, 31801180), Science and Technology Projects in Guangzhou (202201010602, 202201010510), Science and Technology Planning Project of Guangdong Province (2022B1212010010, 2017B030314056, 2018B030306047), Guangdong Basic and Applied Basic Research Foundation (2020A1515110122), National Key R&D Program of China (2017YFA0504100, 2018YFE0204800, 2017YFA0105001, 2018YFA0108700), Guangzhou Regenerative Medicine and Health Guangdong Laboratory project (2018GZR110104003, 2018GZR110105012). The APC was founded by Key Laboratory of Guangdong Higher Education Institutes (2021KSYS009).