Phylogeny and Fatty Acid Profiles of New Pinnularia (Bacillariophyta) Species from Soils of Vietnam

Elena Kezlya; Yevhen Maltsev; Sergei Genkal; Zinaida Krivova; Maxim Kulikovskiy

doi:10.3390/cells11152446

Phylogeny and Fatty Acid Profiles of New Pinnularia (Bacillariophyta) Species from Soils of Vietnam

Cells. 2022 Aug 7;11(15):2446. doi: 10.3390/cells11152446.

Authors

Elena Kezlya¹, Yevhen Maltsev¹, Sergei Genkal², Zinaida Krivova¹, Maxim Kulikovskiy¹

Affiliations

¹ Laboratory of Molecular Systematics of Aquatic Plants, K.A. Timiryazev Institute of Plant Physiology RAS, IPP RAS, 127276 Moscow, Russia.
² Papanin Institute for Biology of Inland Waters RAS, Borok, Nekouz, 152742 Yaroslavl, Russia.

Abstract

We studied the morphology, ultrastructure, and phylogeny of eight soil diatom strains assigned to the Pinnularia genus. Six of these strains, identified by us as new species, are described for the first time. We provide a comprehensive comparison with related species and include ecological data. Molecular phylogeny reconstruction using 18S rDNA and rbcL affiliates the new strains with different subclades within Pinnularia, including 'borealis', 'grunowii' and 'stomatophora'. We also studied the fatty acid profiles in connection with the emerging biotechnological value of diatoms as a source of lipids. Stearic (36.0-64.4%), palmitic (20.1-30.4%), and palmitoleic (up to 20.8%) acids were the dominant fatty acids in the algae cultured on Waris-H + Si medium. High yields of saturated and monounsaturated fatty acids position the novel Pinnularia strains as a promising feedstock for biofuel production.

Keywords: 18S rDNA; biotechnology; diatoms; morphology; rbcL; taxonomy; ultrastructure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Diatoms* / genetics
Diatoms* / ultrastructure
Fatty Acids
Phylogeny
Soil
Vietnam

Substances

Fatty Acids
Soil

Grants and funding

Isolation, morphological and molecular analyses were obtained with financial support by Russian Science Foundation (project number 19-14-00320). Cultivation and fatty acid analysis were obtained with financial support by Russian Science Foundation (project number 20-74-10076). Light and scanning electronic microscopies were obtained within the state assignment of the Ministry of Science and Higher Education of the Russian Federation (theme No. 122042700045-3).