Tensin Regulates Fundamental Biological Processes by Interacting with Integrins of Tonsil-Derived Mesenchymal Stem Cells

Gi Cheol Park; Ji Min Kim; Sung-Chan Shin; Yong-Il Cheon; Eui-Suk Sung; Minhyung Lee; Jin-Choon Lee; Byung-Joo Lee

doi:10.3390/cells11152333

Tensin Regulates Fundamental Biological Processes by Interacting with Integrins of Tonsil-Derived Mesenchymal Stem Cells

Cells. 2022 Jul 29;11(15):2333. doi: 10.3390/cells11152333.

Authors

Gi Cheol Park¹, Ji Min Kim², Sung-Chan Shin², Yong-Il Cheon², Eui-Suk Sung³, Minhyung Lee³, Jin-Choon Lee³, Byung-Joo Lee²

Affiliations

¹ Department of Otolaryngology-Head and Neck Surgery, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon 51353, Korea.
² Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Pusan National University and Biomedical Research Institute, Pusan National University Hospital, Busan 49241, Korea.
³ Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Pusan National University and Biomedical Research Institute, Pusan National University Yangsan Hospital, Yangsan 50612, Korea.

Abstract

Human tonsil-derived mesenchymal stem cells (TMSCs) have a superior proliferation rate and differentiation potential compared to adipose-tissue-derived MSCs (AMSCs) or bone-marrow-derived MSCs (BMSCs). TMSCs exhibit a significantly higher expression of the tensin3 gene (TNS3) than AMSCs or BMSCs. TNS is involved in cell adhesion and migration by binding to integrin beta-1 (ITG β1) in focal adhesion. Here, we investigated the roles of four TNS isoforms, including TNS3 and their relationship with integrin in various biological processes of TMSCs. Suppressing TNS1 and TNS3 significantly decreased the cell count. The knockdown of TNS1 and TNS3 increased the gene and protein expression levels of p16, p19, and p21. TNS1 and TNS3 also have a significant effect on cell migration. Transfecting with siRNA TNS3 significantly reduced Oct4, Nanog, and Sox-2 levels. Conversely, when TNS4 was silenced, Oct4 and Sox-2 levels significantly increase. TNS1 and TNS3 promote osteogenic and adipogenic differentiation, whereas TNS4 inhibits adipogenic differentiation of TMSCs. TNS3 is involved in the control of focal adhesions by regulating integrin. Thus, TNS enables TMSCs to possess a higher proliferative capacity and differentiation potential than other MSCs. Notably, TNS3 plays a vital role in TMSC biology by regulating ITGβ1 activity.

Keywords: differentiation; focal adhesion; proliferation; tensin; tonsil-derived mesenchymal stem cell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Phenomena*
Humans
Integrins / metabolism
Mesenchymal Stem Cells* / metabolism
Palatine Tonsil / cytology*
Palatine Tonsil / metabolism
Tensins / metabolism*

Substances

Integrins
TNS1 protein, human
TNS3 protein, human
Tensins

Grants and funding

This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (no. NRF-2022R1A2C2006697, and NRF2020R1F1A1064763).