A Sulfated Polysaccharide from Red Algae (Gelidium crinale) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells

Haiyan Zheng; Yu Pei; Yuan-Lin He; Yi Liu; Minqi Chen; Pengzhi Hong; Chunxia Zhou; Zhong-Ji Qian

doi:10.3390/foods11152360

A Sulfated Polysaccharide from Red Algae (Gelidium crinale) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells

Foods. 2022 Aug 6;11(15):2360. doi: 10.3390/foods11152360.

Authors

Haiyan Zheng¹, Yu Pei¹, Yuan-Lin He¹, Yi Liu¹, Minqi Chen¹, Pengzhi Hong^{1

2}, Chunxia Zhou^{1

2}, Zhong-Ji Qian^{1

2}

Affiliations

¹ Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, School of Chemistry and Environment, College of Food Science and Technology, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Ocean University, Zhanjiang 524088, China.
² Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524025, China.

Abstract

Sulfated polysaccharides from red algae have a variety of biological activities, especially antitumor activities. Matrix metalloproteinase-9 (MMP-9) is a proteolytic metalloenzyme that degrades the central part of the extracellular matrix (ECM) and promotes tumor metastasis. In this research, we have investigated the influence and mechanism of GNP (sulfated polysaccharide from Gelidium crinale) on tumor metastasis and MMP-9 expression of human fibrosarcoma (HT1080) cells. The results inflected that the concentration of GNP below 100 μg/mL has no toxicity to HT1080 cells, but showed excellent activity in inhibiting cells migration and invasion. In addition, GNP effectively inhibits the mRNA of MMP-9 and reduces its expression and activity by regulating nuclear factor-kappa B (NF-κB), mitogen-activated protein kinases (MAPK) and mTOR/PI3K/Akt signaling pathways. GNP has great potential as MMP-9 inhibitor and could be developed as a functional food or drug to prevent tumor metastasis.

Keywords: Gelidium crinale; MMP-9; invasion; migration; sulfated polysaccharides.

Grants and funding

The research was supported by the Development Project about Marine Economy Demonstration of Zhanjiang City (XM-202008-01B1), the 2020 Shenzhen International Scientific and Technological Cooperation R&D Project (GJHZ20190823111601682), the Natural Science Foundation of Guangdong Province (2020A1515011075), and Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang, ZJW-2019-07).