Expression pattern of NLRC5 in the postnatal mouse brain

Yue Hu; Yi Shen; Xiaojing Wu; Ru Ba; Hongwei Xu; Keze Lu; Yong Shao; Chen Sun; Ying Zhang; Fengqin Miao; Yuqing Shen; Jianqiong Zhang

doi:10.1016/j.acthis.2022.151939

Expression pattern of NLRC5 in the postnatal mouse brain

Acta Histochem. 2022 Oct;124(7):151939. doi: 10.1016/j.acthis.2022.151939. Epub 2022 Aug 8.

Authors

Yue Hu¹, Yi Shen¹, Xiaojing Wu², Ru Ba¹, Hongwei Xu¹, Keze Lu¹, Yong Shao¹, Chen Sun³, Ying Zhang³, Fengqin Miao³, Yuqing Shen⁴, Jianqiong Zhang⁵

Affiliations

¹ Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Southeast University, Nanjing, China.
² Department of Critical Care Medicine, Zhongda Hospital, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine, Southeast University, Nanjing, China.
³ Department of Microbiology and Immunology, Medical School, Southeast University, Nanjing, China.
⁴ Department of Microbiology and Immunology, Medical School, Southeast University, Nanjing, China; Department of Critical Care Medicine, Zhongda Hospital, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine, Southeast University, Nanjing, China.
⁵ Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Southeast University, Nanjing, China; Jiangsu Key Laboratory of Molecular and Functional Imaging, Zhongda Hospital, Medical School, Southeast University, Nanjing, China.

PMID: 35952483
DOI: 10.1016/j.acthis.2022.151939

Abstract

Nucleotide oligomerization domain-like receptors (NLRs), belonging to a large family of pattern recognition receptors, participate in the host's first line of defense against invading pathogens. Caspase recruitment domain containing 5 (NLRC5), the largest member in the NLR family, is demonstrated to be involved in the innate immune response and inflammatory diseases far and wide. Recent studies report that NLRC5 is associated with some central nervous system (CNS) diseases. Besides, NLRC5 is a mastery regulator for the expression of MHC class I both in the immune system and the CNS, while MHC class I is expressed and exerts its function in the brain. Therefore, it is necessary to investigate the expression pattern of NLRC5 in the developing and adult CNS. In our study, postnatal brain sections of C57BL/6 J mice are analyzed for the expression of NLRC5 protein by immunofluorescence. In the postnatal stages of developing telencephalon, NLRC5 exhibits a spatial and temporal expression pattern. NLRC5 is time-specifically expressed in subfields of hippocampus and different layers of prefrontal cortex. Moreover, it is shown that NLRC5 is highly cell type specific. It can be expressed in large quantities by neurons and microglia, but rarely expressed by astrocytes. Taken together, our research is important for further understanding the biological characteristics of NLRC5 and its function in the CNS.

Keywords: Cell type specific; Mouse brain; NLRC5; Spatial and temporal expression pattern.

MeSH terms

Animals
Brain
Immunity, Innate*
Intracellular Signaling Peptides and Proteins* / genetics
Mice
Mice, Inbred C57BL
Nucleotides

Substances

Intracellular Signaling Peptides and Proteins
NLRC5 protein, mouse
Nucleotides