Study of Amyloid Fibers Using Atomic Force Microscopy

Daniel G Cava; Marisela Vélez

doi:10.1007/978-1-0716-2529-3_1

Study of Amyloid Fibers Using Atomic Force Microscopy

Methods Mol Biol. 2022:2538:1-11. doi: 10.1007/978-1-0716-2529-3_1.

Authors

Daniel G Cava¹, Marisela Vélez²

Affiliations

¹ Instituto de Catálisis y Petroleoquímica (CSIC), (Cantoblanco) Madrid, Spain.
² Instituto de Catálisis y Petroleoquímica (CSIC), (Cantoblanco) Madrid, Spain. marisela.velez@icp.csic.es.

PMID: 35951289
DOI: 10.1007/978-1-0716-2529-3_1

Abstract

Atomic force microscopy (AFM) provides high-resolution images of the topography of amyloid fibers adsorbed on surfaces. This information is very useful to study their molecular assembly under various conditions. This chapter describes the basic protocols required to deposit fibers on flat surfaces and discusses some of the practical issues required to operate a good commercial microscope setup to obtain appropriate high-resolution AFM topographic images of amyloid fibers.

Keywords: Amyloid fibers; Atomic force microscopy (AFM); Surfaces; Topographic images.

MeSH terms

Amyloid*
Microscopy, Atomic Force / methods

Substances

Amyloid