Antibiofilm activity of silver nanoparticles biosynthesized using viticultural waste

PLoS One. 2022 Aug 10;17(8):e0272844. doi: 10.1371/journal.pone.0272844. eCollection 2022.

Abstract

Green methods have become vital for sustainable development of the scientific and commercial sphere; however, they can bring new challenges, including the need for detailed characterization and elucidation of efficacy of their products. In this study, green method of silver nanoparticles (AgNPs) production was employed using an extract from grapevine canes. The aim of the study was to contribute to the knowledge about biosynthesized AgNPs by focusing on elucidation of their antifungal efficiency based on their size and/or hypothesized synergy with bioactive substances from Vitis vinifera cane extract. The antifungal activity of AgNPs capped and stabilized with bioactive compounds was tested against the opportunistic pathogenic yeast Candida albicans. Two dispersions of nanoparticles with different morphology (characterized by SEM-in-STEM, DLS, UV-Vis, XRD, and AAS) were prepared by modification of reaction conditions suitable for economical production and their long-term stability monitored for six months was confirmed. The aims of the study included the comparison of the antifungal effect against suspension cells and biofilm of small monodisperse AgNPs with narrow size distribution and large polydisperse AgNPs. The hypothesis of synergistic interaction of biologically active molecules from V. vinifera extracts and AgNPs against both cell forms were tested. The interactions of all AgNPs dispersions with the cell surface and changes in cell morphology were imaged using SEM. All variants of AgNPs dispersions were found to be active against suspension and biofilm cells of C. albicans; nevertheless, surprisingly, larger polydisperse AgNPs were found to be more effective. Synergistic action of nanoparticles with biologically active extract compounds was proven for biofilm cells (MBIC80 20 mg/L of polydisperse AgNPs in extract), while isolated nanoparticles suspended in water were more active against suspension cells (MIC 20 mg/L of polydisperse AgNPs dispersed in water). Our results bring new insight into the economical production of AgNPs with defined characteristics, which were proven to target a specific mode of growth of significant pathogen C. albicans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Antifungal Agents / metabolism
  • Antifungal Agents / pharmacology
  • Biofilms
  • Candida albicans / metabolism
  • Metal Nanoparticles*
  • Microbial Sensitivity Tests
  • Plant Extracts / metabolism
  • Plant Extracts / pharmacology
  • Silver* / metabolism
  • Silver* / pharmacology
  • Water / metabolism

Substances

  • Anti-Bacterial Agents
  • Antifungal Agents
  • Plant Extracts
  • Water
  • Silver

Grants and funding

This work was supported from the grant of Specific university research – grant No. A2_FPBT_2022_018 (UCT Prague), and from the Institutional Support by the Ministry of Industry and Trade. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Funder specifications: Specific university research of University of Chemistry and Technology Prague, grant recipient: A.M., grant number: A2_FPBT_2022_018, full name of funder: Internal Grant Agency, URL of funder: https://www.vscht.cz/research/internal-grant-agency. Institutional Support by the Ministry of Industry and Trade, grant recipient: J.L., grant specifications: Internal project of the Research Centre Řež within the Institutional support from the Ministry of Industry and Trade, Czech Republic, full name of funder: Ministry of Industry and Trade, Czech Republic, URL of the funder: https://www.mpo.cz/.