Toxicity Effects of Engineered Iron Nanoparticles (Fe3O4) on the Growth, Cell Density, and Pigment Content of Chlorella vulgaris

Abstract

This study investigated the effects of Fe₃O₄ nanoparticles released from synthesized thiourea catalyst on the biological aspects of Chlorella vulgaris. Fe₃O₄ concentrations (0, 10, 100, 250, 500, 750, and 1000 mg L^-1) were used for the exposure tests. Biological parameters of C. vulgaris, including cell density, cell viability, and pigment content, were assessed. Bioconcentration and bioaccumulation factors were evaluated for contaminated microalgae. Non-carcinogenic risks were then assessed using target hazard quotient (THQ) for potential risks in human consumptions. Findings showed that C. vulgaris cell number increased from 0 to 500 mg L^-1 of Fe₃O₄ concentration. Chlorophyll a represented a time-dependent response, and greatest values were detected in 250 and 500 mg L^-1 Fe₃O₄ at 4.2 and 4 mg/g, respectively. Chlorophyll b content showed a time-related manner in exposure to Fe₃O₄ with the highest values recorded at 250 mg L^-1 after 96 h. Moreover, bioaccumulation displayed a dose-dependent response at 15,000 µg/g dw in 1000 mg L^-1, whereas the lowest concentration was in the control group at 1700 µg/g dw. The bioconcentration factor showed a concentration-relevant decrease in all iron treatments and 10 mg L^-1 of Fe₃O₄ represented the greatest BCF at 327.3611. Non-carcinogenic risks illustrated negligible hazard (THQ < 1) and the largest EDI and THQ were calculated in 1000 mg L^-1 at 7.4332E-07 (mg kg^-1 day^-1) and 1.06189E-09, respectively. Together, iron is an essential trace element for biological purposes in aquatic systems, but in exceeding concentrations could impose toxicity effects to C. vulgaris populations.