Highly adsorptive protein inorganic nanohybrid of Moringa seeds protein and rice husk nanosilica for effective adsorption of pharmaceutical contaminants

Thi Ngan Vu; Pham Hai Phong Le; Duc Nam Phuong Pham; Thu Ha Hoang; Ashok Kumar Nadda; Thanh Son Le; Tien Duc Pham

doi:10.1016/j.chemosphere.2022.135856

Highly adsorptive protein inorganic nanohybrid of Moringa seeds protein and rice husk nanosilica for effective adsorption of pharmaceutical contaminants

Chemosphere. 2022 Nov;307(Pt 2):135856. doi: 10.1016/j.chemosphere.2022.135856. Epub 2022 Aug 6.

Authors

Thi Ngan Vu¹, Pham Hai Phong Le², Duc Nam Phuong Pham¹, Thu Ha Hoang³, Ashok Kumar Nadda⁴, Thanh Son Le¹, Tien Duc Pham⁵

Affiliations

¹ Faculty of Chemistry, University of Science, Vietnam National University, Hanoi, 19 Le Thanh Tong, Hoan Kiem, Hanoi, 100000, Viet Nam.
² HUS High School for Gifted Students, University of Science, Vietnam National University, Hanoi, 182 Luong the Vinh, Thanh Xuan, Hanoi, 100000, Viet Nam.
³ University of Education, Vietnam National University, Hanoi, 144 Xuan Thuy, Cau Giay, Hanoi, 100000, Viet Nam. Electronic address: hoangthuha0105@vnu.edu.vn.
⁴ Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, Himachal Pradesh, 173 234, India.
⁵ Faculty of Chemistry, University of Science, Vietnam National University, Hanoi, 19 Le Thanh Tong, Hoan Kiem, Hanoi, 100000, Viet Nam. Electronic address: tienduchphn@gmail.com.

PMID: 35944682
DOI: 10.1016/j.chemosphere.2022.135856

Abstract

The present study aims to investigate adsorption characteristics and mechanisms of Moringa (MO) seeds protein on nanosilica rice husk and their applications in removal of pharmaceutical residues including the fluoroquinolone antibiotic levofloxacin (LFX) and the nonsteroidal anti-inflammatory drug diclofenac (DCF) in aquatic environment. Molecular weight of MO protein was determined by gel-permeation chromatography (GPC) method while its amino acids were quantified by high performance liquid chromatography (HPLC). The number-(M_n) and weight-average molecular weights (M_w) of MO protein were 1.53 × 10⁴ and 1.61 × 10⁴ g/mol, respectively. Different effective conditions on adsorption protein on nanosilica including contact time, pH, adsorbent dosage, and ionic strength were systematically optimized and found to be 180 min, 10, 10 mg/mL and 1 mM KCl, respectively. The surface charge change by zeta potential, surface modification by Fourier-transform infrared spectroscopy (FT-IR) and adsorption isotherms demonstrated that protein adsorption on nanosilica was governed by both electrostatic and non-electrostatic interactions. Application of protein functionalized nanosilica (ProFNS) in LFX and DCF removal were also thoroughly studied. The selected conditions for LFX and DCF removal using ProFNS were 1 mM KCl for both LFX and DCF; pH 8 and pH 6; contact time 90 and 120 min, and adsorption dosage 10 and 5 mg/ml for LFX and DCF, respectively. Adsorption isotherms of protein on nanosilica as well as LFX and DCF onto ProFNS at different ionic strengths were reasonably fitted by the two-step model while a pseudo-second-order model could fit adsorption kinetic well. The removal of LFX and DCF using ProFNS significantly increased from 51.51% to 87.35%, and 7.97%-50.02%, respectively. High adsorption capacities of 75.75 mg/g for LFX and 59.52 mg/g for DCF, indicate that ProFNS is a great performance for pharmaceutical residues removal in water environment.

Keywords: Adsorption; Diclofenac; Levofloxacin; Moringa oleifera protein; Nanosilica.

MeSH terms

Adsorption
Amino Acids
Anti-Bacterial Agents
Anti-Inflammatory Agents
Diclofenac / chemistry
Hydrogen-Ion Concentration
Kinetics
Levofloxacin
Moringa*
Oryza*
Pharmaceutical Preparations
Seeds
Spectroscopy, Fourier Transform Infrared
Water
Water Pollutants, Chemical* / chemistry

Substances

Amino Acids
Anti-Bacterial Agents
Anti-Inflammatory Agents
Pharmaceutical Preparations
Water Pollutants, Chemical
Water
Diclofenac
Levofloxacin