Protocol for generation of human peptide-specific primary CD8+ T cell lines

Emma J Grant; Stephanie Gras

doi:10.1016/j.xpro.2022.101590

Protocol for generation of human peptide-specific primary CD8⁺ T cell lines

STAR Protoc. 2022 Jul 31;3(3):101590. doi: 10.1016/j.xpro.2022.101590. eCollection 2022 Sep 16.

Authors

Emma J Grant¹, Stephanie Gras²

Affiliations

¹ Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC 3086, Australia; Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia. Electronic address: e.grant@latrobe.edu.au.
² Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC 3086, Australia; Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia. Electronic address: s.gras@latrobe.edu.au.

Abstract

Directly ex vivo, peptide-specific CD8⁺ T cells are present at relatively low frequency and are typically in a resting state. This protocol details the expansion of memory peptide-specific CD8⁺ T cells by in vitro stimulation, which can be subsequently characterized using a range of assays including tetramer staining and intracellular cytokine staining. For complete details on the use and execution of this protocol, please refer to Lineburg et al. (2021).

Keywords: Cell Biology; Immunology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CD8-Positive T-Lymphocytes* / metabolism
Cell Line
Humans
Peptide T* / metabolism
Peptides / pharmacology

Substances

Peptides
Peptide T