Assessing the Energy Status of Low Numbers of Mammalian Cells by Capillary Electrophoresis-Mass Spectrometry

Wei Zhang; Rawi Ramautar

doi:10.1007/978-1-0716-2493-7_13

Assessing the Energy Status of Low Numbers of Mammalian Cells by Capillary Electrophoresis-Mass Spectrometry

Methods Mol Biol. 2022:2531:203-209. doi: 10.1007/978-1-0716-2493-7_13.

Authors

Wei Zhang¹, Rawi Ramautar²

Affiliations

¹ Biomedical Microscale Analytics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands.
² Biomedical Microscale Analytics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands. r.ramautar@lacdr.leidenuniv.nl.

PMID: 35941487
DOI: 10.1007/978-1-0716-2493-7_13

Abstract

Capillary electrophoresis-mass spectrometry (CE-MS) employing a sheathless porous tip interface has become a strong analytical tool for the efficient profiling of highly polar and charged metabolites in volume/material-restricted biological samples. As more and more metabolomics studies are (intrinsically) dealing with low numbers of mammalian cells, it would be important to use an additional performance metric to effectively evaluate the sampling and sample preparation procedure, in particular quenching. An established parameter to assess the sampling and sample preparation quality when working with cell cultures is the adenylate energy charge (AEC), which represents an index of the energy state of a cell. In this protocol, a CE-MS strategy is proposed for the reliable determination of the adenylate energy charge (AEC) in metabolomics studies dealing with low numbers of mammalian cells.

Keywords: Adenylate energy charge; Biomass-limited samples; Capillary electrophoresis; Mass spectrometry; Metabolic profiling.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Electrophoresis, Capillary* / methods
Mammals
Mass Spectrometry / methods
Metabolomics* / methods
Spectrometry, Mass, Electrospray Ionization / methods