Bacteroides muris sp. nov. isolated from the cecum of wild-derived house mice

Arch Microbiol. 2022 Aug 8;204(9):546. doi: 10.1007/s00203-022-03148-6.

Abstract

Two bacterial strains, KH365_2T and KH569_7, were isolated from the cecum contents of wild-derived house mice. The strains were characterized as Gram-negative, rod-shaped, strictly anaerobic, and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both strains were most closely related to Bacteroides uniformis ATCC 8492T. Whole genome sequences of KH365_2T and KH569_7 strains have a DNA G + C content of 46.02% and 46.03% mol, respectively. Most morphological and biochemical characteristics did not differ between the newly isolated strains and classified Bacteroides strains. However, the average nucleotide identity (ANI) and dDNA-DNA hybridization (dDDH) values clearly distinguished the two strains from described members of the genus Bacteroides. Here, we present the phylogeny, morphology, and physiology of a novel species of the genus Bacteroides and propose the name Bacteroides muris sp. nov., with KH365_2T (DSM 114231T = CCUG 76277T) as type strain.

Keywords: Bacterial microcapsule; Bacteroides muris; Intestinal microbiota; Intraspecific diversity; Murine microbiota; Transmission electron microscopy.

MeSH terms

  • Animals
  • Bacterial Typing Techniques
  • Bacteroides* / genetics
  • Cecum / microbiology
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis
  • Gastropoda*
  • Mice
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S