Dietary Supplementation of Bacillus sp. PM8313 with β-glucan Modulates the Intestinal Microbiota of Red Sea Bream (Pagrus major) to Increase Growth, Immunity, and Disease Resistance

Won Je Jang; Mi-Hyeon Jeon; Su-Jeong Lee; So Young Park; Young-Sun Lee; Da-In Noh; Sang Woo Hur; Seunghan Lee; Bong-Joo Lee; Jong Min Lee; Kang-Woong Kim; Eun-Woo Lee; Md Tawheed Hasan

doi:10.3389/fimmu.2022.960554

Dietary Supplementation of Bacillus sp. PM8313 with β-glucan Modulates the Intestinal Microbiota of Red Sea Bream (Pagrus major) to Increase Growth, Immunity, and Disease Resistance

Front Immunol. 2022 Jul 22:13:960554. doi: 10.3389/fimmu.2022.960554. eCollection 2022.

Authors

Won Je Jang¹, Mi-Hyeon Jeon², Su-Jeong Lee², So Young Park¹, Young-Sun Lee², Da-In Noh², Sang Woo Hur³, Seunghan Lee³, Bong-Joo Lee^{3

4}, Jong Min Lee¹, Kang-Woong Kim³, Eun-Woo Lee^{2

5}, Md Tawheed Hasan^{5

6}

Affiliations

¹ Department of Biotechnology, Pukyong National University, Busan, South Korea.
² Biopharmaceutical Engineering Major, Division of Applied Bioengineering, Dong-Eui University, Busan, South Korea.
³ Aquafeed Research Center, National Institute of Fisheries Science, Pohang, South Korea.
⁴ Department of Smart Fisheries Resources, College of Industrial Sciences, Kongju National University, Yesan, South Korea.
⁵ Core-Facility Center for Tissue Regeneration, Dong-Eui University, Busan, South Korea.
⁶ Department of Aquaculture, Sylhet Agricultural University, Sylhet, Bangladesh.

Abstract

A 56-day feeding trial was conducted to determine the effect of dietary supplementation with Bacillus sp. isolated from the intestines of red sea bream on the growth performance, immunity, and gut microbiome composition of red sea bream. Three diets (a control diet and two treatments) were formulated without Bacillus sp. PM8313 or β-glucan (control, CD), 1 × 10⁸ CFU g^-1 PM8313 (BSD), and 1 × 10⁸ CFU g^-1 PM8313 + 0.1% β-glucan (BGSD). At the end of the experiment, the weight, specific growth rate, feed conversion ratio, and protein efficiency ratio of the fish in the BSD and BGSD diet groups were significantly improved than those of the control group (P < 0.05). Additionally, amylase and trypsin activities were significantly higher (P < 0.05) in both groups compared to the control. Superoxide dismutase and lysozyme activity, which are serum non-specific immune responses, only increased in the BGSD group. The two treatment groups exhibited a marked difference in the intestinal microbiota composition compared to the control group. Furthermore, the treatment groups exhibited an upregulation of IL-6 and NF-κb, coupled with high survival rates when challenged with Edwardsiella tarda. Therefore, dietary supplementation with PM8313 improved the growth performance, digestive enzyme activity, non-specific immunity, and pathogen resistance of red sea bream, in addition to affecting the composition of its intestinal microflora.

Keywords: digestive enzymes; immunity; microbiota; pagrus major; red sea bream.

Publication types

Randomized Controlled Trial, Veterinary
Research Support, Non-U.S. Gov't

MeSH terms

Animal Feed / analysis
Animals
Bacillus*
Dietary Supplements / analysis
Disease Resistance
Gastrointestinal Microbiome*
Perciformes*
Sea Bream*
beta-Glucans* / pharmacology

Substances

beta-Glucans