Paper-based analytical devices, or μPADs, have proven to be valuable bioanalytical tools for a broad range of applications. New methods for μPAD fabrication are needed, however, to facilitate their mass production at a competitive cost. To address this need, we report the use of a boronic acid-containing siloxane polymer (BorSilOx) for patterning hydrophobic barriers for μPADs. This material functions by covalently binding to hydroxyl groups in the paper substrate. It is compatible with inkjet printing or roll-to-roll (stamping) processes, as demonstrated here using three different deposition methods. BorSilOx is able to render a broad range of cellulosic materials (from paper towels to wood) hydrophobic, with contact angle measurements demonstrating superhydrophobicity in many cases. We further demonstrate the utility of the polymer in μPADs via assays for pH and glucose.
Keywords: assay manufacturing; hydrophobic patterning; paper-based microfluidics; μPADs.