Changes in hydration of liposome membranes exposed to nanosecond electric pulses detected by wide-field Coherent anti-Stokes Raman microspectroscopy

Caterina Merla; Martina Nardoni; Michael Scherman; Stefania Petralito; Laura Caramazza; Francesca Apollonio; Micaela Liberti; Patrizia Paolicelli; Brigitte Attal-Tretout; Lluis M Mir

doi:10.1016/j.bioelechem.2022.108218

Changes in hydration of liposome membranes exposed to nanosecond electric pulses detected by wide-field Coherent anti-Stokes Raman microspectroscopy

Bioelectrochemistry. 2022 Oct:147:108218. doi: 10.1016/j.bioelechem.2022.108218. Epub 2022 Jul 30.

Authors

Affiliations

¹ Université Paris-Saclay, CNRS, Gustave Roussy, Metabolic and Systemic Aspects of Oncogenesis (METSY), 114 rue Édouard-Vaillant, 94805 Villejuif Cedex, France. Electronic address: caterina.merla@enea.it.
² Department of Drug Chemistry and Technologies, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
³ ONERA, BP 80100 FR-91123, Palaiseau Cedex, France. Electronic address: michael.scherman@onera.fr.
⁴ Department of Drug Chemistry and Technologies, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy. Electronic address: stefania.petralito@uniroma1.it.
⁵ Department of Information Engineering Electronic and Telecommunications, Sapienza University of Rome, via Eudossiana 18, 00184 Rome, Italy. Electronic address: laura.caramazza@uniroma1.it.
⁶ Department of Information Engineering Electronic and Telecommunications, Sapienza University of Rome, via Eudossiana 18, 00184 Rome, Italy. Electronic address: francesca.apollonio@uniroma1.it.
⁷ Department of Information Engineering Electronic and Telecommunications, Sapienza University of Rome, via Eudossiana 18, 00184 Rome, Italy. Electronic address: micaeal.liberti@uniroma1.it.
⁸ Department of Drug Chemistry and Technologies, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy. Electronic address: patrizia.paolicelli@uniroma1.it.
⁹ ONERA, BP 80100 FR-91123, Palaiseau Cedex, France. Electronic address: brigitte.attal-tretout@onera.fr.
¹⁰ Université Paris-Saclay, CNRS, Gustave Roussy, Metabolic and Systemic Aspects of Oncogenesis (METSY), 114 rue Édouard-Vaillant, 94805 Villejuif Cedex, France. Electronic address: luis.mir@cnrs.fr.

PMID: 35933972
DOI: 10.1016/j.bioelechem.2022.108218

Abstract

Electropulsation has become a powerful technological platform for electromanipulation of cells and tissues for various medical and biotechnological applications, but the molecular changes that underlay the very first initiation step of this process have not been experimentally observed. Here, we endowed a wide-field Coherent anti-Stokes Raman Scattering platform with an ad-hoc electromagnetic exposure device and we demonstrated, using artificial lipid vesicles (i.e. liposomes), that electropulsation is initiated by the increase of interstitial water content in liposome membranes. A pulse-dependent accumulation of the interstitial water molecules is observed in the membranes and a plausible mechanism supported by a computational electrochemical model is presented and discussed.

Keywords: Coherent anti-Stokes Raman Scattering; Interfacial water molecules; Interstitial water molecules; Liposomes; Nanosecond pulsed electric fields.

MeSH terms

Electricity
Liposomes*
Spectrum Analysis, Raman* / methods
Water

Substances

Liposomes
Water