ASu@MNPs-based electrochemical immunosensor for vitamin D3 serum samples analysis

Francesca Polli; Cristine D'Agostino; Rosaceleste Zumpano; Viviana De Martino; Gabriele Favero; Luciano Colangelo; Salvatore Minisola; Franco Mazzei

doi:10.1016/j.talanta.2022.123755

ASu@MNPs-based electrochemical immunosensor for vitamin D₃ serum samples analysis

Talanta. 2023 Jan 1:251:123755. doi: 10.1016/j.talanta.2022.123755. Epub 2022 Aug 2.

Authors

Francesca Polli¹, Cristine D'Agostino¹, Rosaceleste Zumpano¹, Viviana De Martino², Gabriele Favero³, Luciano Colangelo², Salvatore Minisola², Franco Mazzei⁴

Affiliations

¹ Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.
² Department of Clinical, Internal, Anaesthesiologic and Cardiovascular Sciences, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.
³ Department of Environmental Biology, Sapienza University of Rome, P.le Aldo Moro 5, Rome, 00185, Italy.
⁴ Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy. Electronic address: franco.mazzei@uniroma1.it.

PMID: 35932635
DOI: 10.1016/j.talanta.2022.123755

Abstract

We report a new sensitive label-free electrochemical immunosensor to detect Vitamin D₃ (25-OHD₃) in untreated serum samples. To this aim, a graphite screen printed electrode (SPE) was modified using cysteamine (CYM) functionalized core-shell magnetic nanoparticles (Au@MNPs) then, the 25-OHD₃ antibody (AbD) was immobilized via glutaraldehyde crosslinking. The several steps involved in the immunosensor development and 25-OHD₃ analysis were monitored by using differential pulse voltammetry (DPV). The developed immunosensor showed a LOD of 2.4 ng mL^-1 and a linear range between 7.4 and 70 ng mL^-1. The effectiveness of the immunosensor in human serum analysis was assessed by comparing the results obtained with the chemiluminescence-immunoassay (CLIA) reference method. The high sensitivity and excellent agreement with the reference method suggest its potential use as a POCT to monitor hypovitaminosis 25-OHD levels.

Keywords: Differential pulse voltammetry; Gold-magnetic nanoparticles; Label-free immunosensor; Vitamin D(3) detection.

MeSH terms

Biosensing Techniques* / methods
Cholecalciferol
Cysteamine
Electrochemical Techniques / methods
Electrodes
Glutaral
Gold
Graphite*
Humans
Immunoassay / methods
Limit of Detection
Metal Nanoparticles*

Substances

Cholecalciferol
Cysteamine
Gold
Graphite
Glutaral