BCLAF1, a functional partner of BACH1, participates in DNA damage response

Abstract

BACH1 (Brca1-Associated C-terminal Helicase) is an important DNA damage response factor, which is involved in DNA damage repair and maintenance of genomic stability. In this study, by using tandem protein affinity purification, we have identified BCLAF1 as a novel functional partner of BACH1. BCLAF1 constitutively interacts with BACH1 regardless of DNA damage. However, in response to DNA damage, along with BACH1, BCLAF1 is recruited to the DNA damage sites and the recruitment of BCLAF1 was regulated by BACH1 and BRCA1. Interestingly, BCLAF1 deficient cells are deficient for DSB-initiated HR, but RAD51 foci formation is intact following IR treatment. Taken together, these findings reveal that BCLAF1 is a functional binding partner of BACH1 playing a key role in DNA damage response.

Keywords: BACH1; BCLAF1; DNA damage response; Homologous recombination repair.