Light-induced stabilization of microtubules by photo-crosslinking of a Tau-derived peptide

Soei Watari; Hiroshi Inaba; Tomonori Tamura; Arif Md Rashedul Kabir; Akira Kakugo; Kazuki Sada; Itaru Hamachi; Kazunori Matsuura

doi:10.1039/d2cc01890j

Light-induced stabilization of microtubules by photo-crosslinking of a Tau-derived peptide

Chem Commun (Camb). 2022 Aug 16;58(66):9190-9193. doi: 10.1039/d2cc01890j.

Authors

Soei Watari¹, Hiroshi Inaba^{1

2}, Tomonori Tamura³, Arif Md Rashedul Kabir⁴, Akira Kakugo^{4

5}, Kazuki Sada^{4

5}, Itaru Hamachi^{3

6}, Kazunori Matsuura^{1

2}

Affiliations

¹ Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan. hinaba@tottori-u.ac.jp.
² Centre for Research on Green Sustainable Chemistry, Tottori University, Tottori 680-8552, Japan.
³ Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
⁴ Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan.
⁵ Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo 060-0810, Japan.
⁶ JST-ERATO, Hamachi Innovative Molecular Technology for Neuroscience, Nishikyo-ku, Kyoto 615-8530, Japan.

PMID: 35929838
DOI: 10.1039/d2cc01890j

Abstract

For light-induced stabilization of microtubules (MTs) to manipulate cells, a photo-reactive diazirine group was conjugated to a Tau-derived peptide, a motif binding on the inside of MTs. Ultraviolet (UV) light irradiation induced significant stabilization of MTs via the formation of a covalent bond of the peptide and showed toxicity.

MeSH terms

Microtubules* / metabolism
Peptides / metabolism
Ultraviolet Rays
tau Proteins* / metabolism

Substances

Peptides
tau Proteins