circRPPH1_025 Overexpression Promotes Migration and Invasion of Glioblastoma Multiforme

Lixiong Xue; Huahui Chen; Xiaolong Wang; Li Han; Yifan Liu; Xinmin Ding

doi:10.1155/2022/4764028

circRPPH1_025 Overexpression Promotes Migration and Invasion of Glioblastoma Multiforme

Dis Markers. 2022 Jul 26:2022:4764028. doi: 10.1155/2022/4764028. eCollection 2022.

Authors

Lixiong Xue^{1

2}, Huahui Chen^{1

2}, Xiaolong Wang^{1

2}, Li Han^{1

2}, Yifan Liu^{1

2}, Xinmin Ding^{1

2}

Affiliations

¹ Department of Neurosurgery, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan 030032, China.
² Department of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Abstract

Objective: To study the effect of circ_0000512 (circRPPH1_025) on the tumorigensis and development of glioblastoma and its molecular mechanism.

Methods: The expression levels of circ_0000512 in normal astrocytes (NHA) and human glioblastoma cell lines (U87, U251, and A172) and the expression levels of circ_0000512 and linear RNA RPPH1 in U87 cells after RNase R treatment were detected by qRT-PCR. The effects of circ_0000512 knockdown or overexpression on the proliferation, migration, invasion, and epithelial-mesenchymal transition of U87 cells were detected by CCK-8 assay, cell colony formation assay, transwell invasion assay, wound healing assay, and western blot.

Results: The expression of circ_0000512 was upregulated in glioblastoma cells, and the overexpression of circ_0000512 was beneficial to the proliferation, migration, invasion, and epithelial-mesenchymal transition of U87 cells, while knockdown of circ_0000512 showed the opposite results.

Conclusion: circ_0000512 can be used as a potential target for early diagnosis and targeted therapy of glioblastoma multiforme.

MeSH terms

Brain Neoplasms* / genetics
Brain Neoplasms* / metabolism
Cell Line, Tumor
Cell Movement / genetics
Cell Proliferation / genetics
Glioblastoma* / genetics
Glioblastoma* / metabolism
Humans
MicroRNAs* / genetics
RNA, Circular / genetics

Substances

MicroRNAs
RNA, Circular