Probiotic Lactobacillus rhamnosus GR-1 supplementation attenuates Pb-induced learning and memory deficits by reshaping the gut microbiota

Xiaozhen Gu; Nanxi Bi; Tian Wang; Chengqing Huang; Rongrong Wang; Yi Xu; Hui-Li Wang

doi:10.3389/fnut.2022.934118

Probiotic Lactobacillus rhamnosus GR-1 supplementation attenuates Pb-induced learning and memory deficits by reshaping the gut microbiota

Front Nutr. 2022 Jul 19:9:934118. doi: 10.3389/fnut.2022.934118. eCollection 2022.

Authors

Xiaozhen Gu^{1

2}, Nanxi Bi^{1

2}, Tian Wang^{1

2}, Chengqing Huang^{1

2}, Rongrong Wang^{1

2}, Yi Xu^{1

2}, Hui-Li Wang^{1

2}

Affiliations

¹ Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei, China.
² School of Food and Biological Engineering, Hefei University of Technology, Hefei, China.

Abstract

Lead (Pb) exposure during early life has been associated with an increased risk of neurodevelopmental disorders, including learning and memory deficits. The intestinal flora, via the microbiome-gut-brain axis, could play a significant role in the nervous system. However, the effects of probiotics on ameliorating Pb-induced learning and memory deficits are still unclear. In this study, we showed that adolescent Pb exposure (150 ppm) for 2 months impaired spatial learning and memory ability, accompanied by the decreasing diversity of gut microbiota, and the decreasing abundance of Lactobacillus at the genus level. Surprisingly, administration of the Lactobacillus rhamnosus GR-1 (10¹⁰ organisms/rat/day), not L. rhamnosus LGG or Lactobacillus reuteri RC-14, reversed learning and memory deficits induced by Pb exposure. Meanwhile, administration of the L. rhamnosus GR-1 increased the diversity of the gut microbiota composition and partially normalized the genus level of Lactobacillus, Parabacteroides, Enterococcus, and Akkermansia in Pb-exposed rats. Notably, supplementation of L. rhamnosus GR-1 decreased the gut permeability of Pb-exposed rats, reduced proinflammatory cytokines [interleukin-1β (IL-1β) and IL-6] expression, and promoted anti-inflammatory cytokines [granulocyte colony-stimulating factor (G-CSF)] expression. Interestingly, neural cell treatment with G-CSF rescued Pb-induced neurotoxicity. In general, L. rhamnosus GR-1 supplementation recovered the Pb-induced loss of intestinal bacteria (Lactobacillus), which may have reversed the damage to learning and memory ability. Collectively, our findings demonstrate an unexpectedly pivotal role of L. rhamnosus GR-1 in Pb-induced cognitive deficits and identify a potential probiotic therapy for cognitive dysfunction during early life.

Keywords: G-CSF; Lactobacillus rhamnosus; Pb exposure; gut microbiota; learning and memory deficits.