Poultry meat and eggs are a primary source of animal protein. To meet the market needs, high yield laying hens are reared continuously, resulting in quick ovary aging. Thus, we investigated the anti-aging effects of Shudi Erzi San (SES) on laying hens. Sixty 300-day-old laying hens were divided into 2 experimental groups and a control group. The control group was fed on a basic diet, which was supplemented with 1% and 2% SES for experimental groups I and II, respectively. Egg quality and changes in serum hormones and blood-biochemical indicators of laying hens were determined. The rate of egg production was significantly higher in group Ⅱ than in both the control and group Ⅰ by 9.29 and 8.22 percentage points, respectively (P < 0.05). Eggshell strength of groups Ⅰ and Ⅱ were significantly higher than that of the control group (P < 0.01). Albumen height and Haugh Units of group Ⅱ were significantly higher than those of the control (P < 0.05). Serum levels of follicle stimulating hormone and estradiol in group Ⅱ were significantly higher than those of both the control and group Ⅰ (P < 0.05), whereas groups Ⅰ and Ⅱ had significantly higher serum levels of luteinizing hormone than the control (P < 0.05). Levels of superoxide dismutase (SOD) did not significantly differ between the control and group Ⅰ (P > 0.05), but SOD and malondialdehyde (MDA) levels in group Ⅱ were significantly higher and lower, respectively (P < 0.05) when compared to the control. Compared with the control, uric acid levels in groups Ⅰ and Ⅱ were significantly lower (P < 0.05), as was urea nitrogen in group Ⅱ (P < 0.05). Transcriptome and KEGG pathway analysis of ovarian tissues of laying hens showed a significant immune related signal pathway as the possible main regulator of a lysosome related signal pathway. Thus, supplementing chicken feed with SES improves egg production and quality and alleviates ovarian decline in laying hens.
Keywords: Shudi Erzi San; aging; antioxidants; laying hens; ovary.
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