Evaluation of Proton MR Spectroscopy for the Study of the Tongue Tissue in Healthy Subjects and Patients With Tongue Squamous Cell Carcinoma: Preliminary Findings

Salem Boussida; Yvener François; Adrien Heintz; Zuzana Saidak; Stéphanie Dakpé; Alexandre Coutte; Bruno Chauffert; Bernard Devauchelle; Antoine Galmiche; Sylvie Testelin; Patrick Goudot; Jean-Marc Constans

doi:10.3389/froh.2022.912803

Evaluation of Proton MR Spectroscopy for the Study of the Tongue Tissue in Healthy Subjects and Patients With Tongue Squamous Cell Carcinoma: Preliminary Findings

Front Oral Health. 2022 Jul 18:3:912803. doi: 10.3389/froh.2022.912803. eCollection 2022.

Authors

Salem Boussida^{1

2}, Yvener François³, Adrien Heintz^{1

2}, Zuzana Saidak^{2

4}, Stéphanie Dakpé^{2

5}, Alexandre Coutte^{2

6}, Bruno Chauffert⁷, Bernard Devauchelle^{2

5}, Antoine Galmiche^{2

4}, Sylvie Testelin^{2

5}, Patrick Goudot³, Jean-Marc Constans^{1

2}

Affiliations

¹ Radiology Department, University Hospital of Amiens Picardie, Amiens, France.
² CHIMERE UR 7516 Research Team for Head & Neck, Institute Faire Faces, University of Picardie Jules Verne, Amiens, France.
³ Faculty of Medicine, Assistance Publique - Hôpitaux de Paris (AP-HP), Sorbonne University, Paris, France.
⁴ Department of Biochemistry, University Hospital of Amiens, Amiens, France.
⁵ Department of Maxillofacial Surgery and Stomatology, University Hospital of Amiens, Amiens, France.
⁶ Department of Radiotherapy, University Hospital of Amiens, Amiens, France.
⁷ Department of Medical Oncology, University Hospital of Amiens, Amiens, France.

Abstract

Purpose: To noninvasively assess spectroscopic and metabolic profiles of healthy tongue tissue and in an exploratory objective in nontreated and treated patients with tongue squamous cell carcinoma (SCC).

Methods: Fourteen healthy subjects (HSs), one patient with nontreated tongue SCC (NT-SCC), and two patients with treated tongue SCC (T-SCC) underwent MRI and single-voxel proton magnetic resonance spectroscopy (¹H-MRS) evaluations (3 and 1.5T). Multi-echo-times ¹H-MRS was performed at the medial superior part (MSP) and the anterior inferior part (AIP) of the tongue in HS, while ¹H-MRS voxel was placed at the most aggressive part of the tumor for patients with tongue SCC. ¹H-MRS data analysis yielded spectroscopic metabolite ratios quantified to total creatine.

Results: In HS, compared to MSP and AIP, ¹H-MRS spectra revealed higher levels of creatine, a more prominent and well-identified trimethylamine-choline (TMA-Cho) peak. However, larger prominent lipid peaks were better differentiated in the tongue MSP. Compared to HS, patients with NT-SCC exhibited very high levels of lipids and relatively higher values of TMA-Cho peak. Interestingly, patients with T-SCC showed almost nonproliferation activity. However, high lipids levels were measured, although they were relatively lower than lipids levels measured in patients with NT-SCC.

Conclusion: The present study demonstrated the potential use of in-vivo ¹H-MRS to noninvasively assess spectroscopic and metabolic profiles of the healthy tongue tissue in a spatial location-dependent manner. Preliminary results revealed differences between HS and patients with tongue NT-SCC as well as tongue T-SCC, which should be confirmed with more patients. ¹H-MRS could be included, in the future, in the arsenal of tools for treatment response evaluation and noninvasive monitoring of patients with tongue SCC.

Keywords: 1H-MRS; noninvasive biomarkers; proton magnetic resonance spectroscopy; spectroscopic and metabolic profile; squamous cell carcinoma; tongue tissue.