Gelatin-hydroxyapatite nano-fibers as promising scaffolds for guided tissue regeneration (GTR): Preparation, assessment of the physicochemical properties and the effect on mesenchymal stem cells

Simin Sharifi; Mohammad Samiei; Elaheh Dalir Abdolahinia; Rovshan Khalilov; Shahriar Shahi; Solmaz Maleki Dizaj

doi:10.34172/japid.2020.001

Gelatin-hydroxyapatite nano-fibers as promising scaffolds for guided tissue regeneration (GTR): Preparation, assessment of the physicochemical properties and the effect on mesenchymal stem cells

J Adv Periodontol Implant Dent. 2020 Apr 8;12(1):25-29. doi: 10.34172/japid.2020.001. eCollection 2020.

Authors

Simin Sharifi¹, Mohammad Samiei^{2

3}, Elaheh Dalir Abdolahinia⁴, Rovshan Khalilov^{5

6

7}, Shahriar Shahi^{1

2}, Solmaz Maleki Dizaj¹

Affiliations

¹ Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
² Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
³ Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
⁴ Research Center of Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
⁵ Department of Biophysics and Molecular Biology, Baku State University, Baku, Azerbaijan.
⁶ Institute of Radiation Problems, National Academy of Sciences of Azerbaijan, Baku, Azerbaijan.
⁷ International Research and Education Center of Nanobiotechnology and Functional Nanosystems, Drohobych Ukraine & Baku, Azerbaijan.

Abstract

Background: Periodontitis can lead to progressive destruction of periodontal tissues supporting the tooth. Developing biomaterials for tissue engineering has noticeably improved the existing treatment options. The present study investigated the gelatin-hydroxyapatite nano-fibers as promising scaffolds for guided tissue regeneration (GTR).

Methods: The scaffolds were prepared through electrospinning technique, and then the physicochemical properties and the cytotoxic effects on dental-derived mesenchymal stem cells were assessed.

Results: The nano-scaffolds were successfully prepared with a mono-dispersed nano-scale diameter (102±0.10 nm), negative surface charge (-20±0.17 mV), and uniform network-shaped morphology. The mesenchymal stem cells derived from the human dental pulp stem cells (hDPSC) with gelatin-hydroxyapatite nano-fibers showed that the prepared scaffolds had a significant proliferative effect. Besides, the applied method can be used to prepare fiber-based structures via other polymeric materials.

Conclusion: The incorporation of different materials to decrease the degradation rate of the fibers can match the speed of tissue regeneration. In this case, the prepared nano-fibers can be applied as a membrane biomaterial.

Keywords: GTR; Gelatin; Hydroxyapatite; Nano-scaffolds; Stem cell.

Grants and funding

Funding: This study was supported by Vice Chancellor for Research at Tabriz University of Medical Sciences.