CRISPR/Cas9-mediated generation of non-motile mutants to improve the harvesting efficiency of mass-cultivated Euglena gracilis

Plant Biotechnol J. 2022 Nov;20(11):2042-2044. doi: 10.1111/pbi.13904. Epub 2022 Sep 8.

Marumi Ishikawa¹, Toshihisa Nomura^{1

2}, Shun Tamaki¹, Kazunari Ozasa³, Tomoko Suzuki^{4

5}, Kiminori Toyooka⁴, Kikue Hirota¹, Koji Yamada^{1

6}, Kengo Suzuki^{1

6}, Keiichi Mochida^{1

2

7

8

9}

¹ Microalgae Production Control Technology Laboratory, RIKEN Baton Zone Program, RIKEN Cluster for Science, Technology and Innovation Hub, Yokohama, Japan.
² Bioproductivity Informatics Research Team, RIKEN Center for Sustainable Resource Science, Yokohama, Japan.
³ Advanced Laser Processing Research Team, RIKEN Center for Advanced Photonics, Wako, Japan.
⁴ Mass Spectrometry and Microscopy Unit, Technology Platform Division, RIKEN Center for Sustainable Resource Science, Kanagawa, Japan.
⁵ Center for Gene Research, Nagoya University, Aichi, Japan.
⁶ euglena Co., Ltd., Tokyo, Japan.
⁷ Kihara Institute for Biological Research, Yokohama City University, Yokohama, Japan.
⁸ Graduate School of Nanobioscience, Yokohama City University, Yokohama, Japan.
⁹ School of Information and Data Sciences, Nagasaki University, Nagasaki, Japan.

No abstract available

Keywords: Euglena gracilis; Bardet-Biedl syndrome (BBS) genes; Cas9 ribonucleoprotein (RNP)-based genome editing; harvesting efficiency.

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