Effect of pre-conceptional nutrition and season on fetal growth during early pregnancy in sheep

Abstract

Gestational age in sheep can be closely predicted through ultrasonographic measurement of fetal bones when correlated to standardized fetal growth curves. However, these standardized curves do not account for factors that are known modulators of fetal growth, such as maternal nutrition or health status. Despite being seasonal breeders, and studies reporting an effect of season on birth weight, the influence of season on fetal growth has not been well characterized. In this study, we hypothesized that season of conception will affect fetal growth curves during mid-gestation and that pre-conceptional nutrition would have no effect. We investigated this by provisioning treatments of low, control, and high planes of nutrition during the lactation and flushing pre-conceptional periods to multiparous Dorset x Polypay and Dorset ewes over two seasons (the optimal breeding season [n = 97] and the suboptimal breeding season [n = 104]). Females were mated naturally with mating dates recorded, fetal biparietal diameter measured via ultrasound between gestational days 35-71, and newborn weights recorded at lambing. Pre-conceptional nutritional treatments did not affect fetal biparietal diameter. However, low vs. high nutrition in the pre-conceptional lactation (but not flushing) period resulted in reduced lamb birth weights (P < 0.001). Early fetal growth tended to be faster in the suboptimal breeding season than in the optimal breeding season (P < 0.061) with lambs being heavier at birth in the optimal breeding season (P < 0.001). There was no effect of fetal sex or litter size on fetal biparietal diameter during the first half of pregnancy, however both sex and litter size influenced lamb birth weight (P < 0.001) with males being heavier than females and singletons being heavier than twins and triplets. Mating date within the flushing period had a significant effect on lamb birth weight regardless of season and independent of treatment, with ewes that conceived later in the flushing period having heavier lambs at birth (P = 0.007). These findings suggest that pre-conceptional under- or overnutrition resulting in substantial changes in body condition does not affect fetal growth during the first half of pregnancy. However, the reduction in lamb birth weight indicates that pre-conceptional maternal nutrition during the previous lactation period may affect fetal growth later in pregnancy.

Keywords: Fetal growth; Pre-conceptional nutrition; Seasonality; Sheep; Ultrasonography.