Genome-wide identification of long non-coding (lncRNA) in Nilaparvata lugens's adaptability to resistant rice

PeerJ. 2022 Jul 25:10:e13587. doi: 10.7717/peerj.13587. eCollection 2022.

Abstract

Background: The brown planthopper (BPH), Nilaparvata lugens (Stål), is a very destructive pest that poses a major threat to rice plants worldwide. BPH and rice have developed complex feeding and defense strategies in the long-term co-evolution.

Methods: To explore the molecular mechanism of BPH's adaptation to resistant rice varieties, the lncRNA expression profiles of two virulent BPH populations were analyzed. The RNA-seq method was used to obtain the lncRNA expression data in TN1 and YHY15.

Results: In total, 3,112 highly reliable lncRNAs in TN1 and YHY15 were identified. Compared to the expression profiles between TN1 and YHY15, 157 differentially expressed lncRNAs, and 675 differentially expressed mRNAs were identified. Further analysis of the possible regulation relationships between differentially expressed lncRNAs and differentially expressed mRNAs, identified three pair antisense targets, nine pair cis-regulation targets, and 3,972 pair co-expressed targets. Function enriched found arginine and proline metabolism, glutathione metabolism, and carbon metabolism categories may significantly affect the adaptability in BPH when it is exposed to susceptible and resistant rice varieties. Altogether, it provided scientific data for the study of lncRNA regulation of brown planthopper resistance to rice. These results are helpful in the development of new control strategies for host defense against BPH and breeding rice for high yield.

Keywords: Adaptability; Co-expression; Nilaparvata lugens; Regulation; lncRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Gene Expression Profiling
  • Hemiptera* / genetics
  • Oryza* / genetics
  • Plant Breeding
  • RNA, Long Noncoding* / genetics

Substances

  • RNA, Long Noncoding

Grants and funding

This work was funded by the National Key Research and Development Program of China (No. 2021YFD1401100), and the National Natural Science Foundation of China (No. 31501654). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.