The diversity of bacterial endophytes from Iris pseudacorus L . and their plant beneficial traits

Vyacheslav Shurigin; Jakhongir Alimov; Kakhramon Davranov; Tashkhan Gulyamova; Dilfuza Egamberdieva

doi:10.1016/j.crmicr.2022.100133

The diversity of bacterial endophytes from Iris pseudacorus L . and their plant beneficial traits

Curr Res Microb Sci. 2022 Apr 12:3:100133. doi: 10.1016/j.crmicr.2022.100133. eCollection 2022.

Authors

Vyacheslav Shurigin^{1

2}, Jakhongir Alimov¹, Kakhramon Davranov², Tashkhan Gulyamova², Dilfuza Egamberdieva¹

Affiliations

¹ Faculty of Biology, National University of Uzbekistan, Tashkent, 100174, Uzbekistan.
² Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan, Tashkent, 100128, Uzbekistan.

Abstract

This study reports the diversity of cultivable endophytic bacteria associated with yellow iris (Iris pseudacorus L.) by using 16S rRNA gene analysis and their plant beneficial traits. The 16S rRNA sequence similarities of endophytic bacteria isolated from the leaves and roots of yellow iris showed that the isolates belonged to the genera Staphylococcus, Streptomyces, Variovorax, Pantoea, Paenibacillus, Bacillus, Janthinobacterium, Enterobacter, Brevibacterium, Agrobacterium, Rhizobium, Xanthomonas translucens, and Pseudomonas. The endophytic bacteria Pseudomonas gessardii HRT18, Brevibacterium frigoritolerans HRT8, Streptomyces atratus HRT13, and Bacillus toyonensis HST13 exhibited antimicrobial activity against five plant pathogenic fungi Fusarium, Rhizoctonia, Botrytis, Pythium, and Alternaria. They also demonstrated the capability to produce chitinase, protease, glucanase, lipase, HCN, and indole-3-acetic acid (IAA). Thirteen isolates (46%) produced IAA, and the most active IAA producers were Bacillus cereus, Agrobacterium tumefaciens, Agrobacterium vitis, Bacillus megaterium, and Bacillus aryabhattai. The IAA producing bacterial isolates stimulated root and shoot growth of garden cress. Our findings suggest that medicinal plants could be a promising source for isolating plant-beneficial bacteria that can be used to enhance the growth and protect plants against soil-borne pathogens.

Keywords: Endophytes; Plant beneficial traits; Plant pathogenic fungi; Yellow iris.