Research Note: In-feed Bacteriophage does not impact efficacy of live Salmonella vaccine

Abstract

Dietary bacteriophages potentially can serve as a step to reduce Salmonella contamination of feed through direct lysis of the bacteria. However, poultry producers commonly vaccinate with live Salmonella vaccines, which could potentially be lysed by dietary bacteriophages. The objective of this study was to evaluate if dietary bacteriophages impacted the colonization of a live Salmonella vaccine. A total of 210 day-of-hatch Ross male broiler chicks were divided into 3 treatments consisting of 2 replicate per treatment. Each replicate contained 35 birds. T1 was the challenge control, given no Salmonella vaccine, T2 was challenged and given Salmonella vaccine and T3 was challenged, given Salmonella vaccine as well as dietary bacteriophage. Salmonella vaccine was administered day of hatch. On d 3, four birds/pen were sampled for Salmonella vaccine colonization of ceca and liver/spleen. The remaining birds were challenged with 5 × 10⁷ CFU of nalidixic acid- resistant Salmonella enteritidis (S.E.). On d 28, ten birds/replicate were sampled via cloaca swabs to culture for S.E. On d 42, the trial was terminated, birds were weighed, and performance was calculated. In addition, 15 birds/replicate were sampled for cecal cultures of S.E. On d 3, T1 had 0% vaccine strain isolated, and significantly lower (P = 0.009) cecal prevalence compared with T2 (75%) and T3 (38%) being intermediate. T1 (0%) had significantly lower liver/spleen vaccine strain prevalence (P = 0.002) compared with T3 (88%) and T2 (63%) being intermediate. No significant differences (P > 0.05) were observed among treatments in Salmonella prevalence in d 28 cloacal swabs. No significant differences (P > 0.05) were observed in d 42 cecal Salmonella prevalence between all treatments. No significant differences in bird weight were observed between treatments d 0 to 42 (P > 0.05). However, T2 and T3 had lower mortality and adjusted feed conversion ratio (FCR; P < 0.05) compared with T1. Therefore, the dietary bacteriophage did not interfere with colonization or protection afforded by the live Salmonella vaccine.

Keywords: ABF; Salmonella; Salmonella vaccine; bacteriophage; food Safety.