Preparation of Fe3O4@Fe(0) immobilized enzyme to enhance the efficient degradation of methoxychlor

He Bai; Yuxiang Yang; Hongming Yuan; Xiangnong Liu; Chaoying Ni

doi:10.1007/s11356-022-22265-9

Preparation of Fe₃O₄@Fe(0) immobilized enzyme to enhance the efficient degradation of methoxychlor

Environ Sci Pollut Res Int. 2023 Jan;30(1):917-929. doi: 10.1007/s11356-022-22265-9. Epub 2022 Jul 30.

Authors

He Bai¹, Yuxiang Yang^{2

3}, Hongming Yuan⁴, Xiangnong Liu⁵, Chaoying Ni⁶

Affiliations

¹ School of Chemistry and Molecular Engineering, East China University of Science & Technology, Shanghai, 200237, China.
² School of Chemistry and Molecular Engineering, East China University of Science & Technology, Shanghai, 200237, China. yxyang@ecust.edu.cn.
³ Department of Materials Science and Engineering, University of Delaware, Newark, DE, 19716, USA. yxyang@ecust.edu.cn.
⁴ State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University, Changchun, 130012, China.
⁵ Analysis Test Center, Yangzhou University, Yangzhou, 225009, China.
⁶ Department of Materials Science and Engineering, University of Delaware, Newark, DE, 19716, USA.

PMID: 35908032
DOI: 10.1007/s11356-022-22265-9

Abstract

The presence of methoxychlor (MXC) in soil and wastewater is considered a nonnegligible environmental threat. Herein, Fe₃O₄@Fe(0) was obtained by NaBH₄ reduction of Fe₃O₄ nanoparticles and served as a carrier for laccase to construct catalyst. The catalyst was evaluated for the degradation of MXC in treated wastewater and soil with 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) being used as cocatalyst. The removal rate of MXC in wastewater and soil was found to be 89% and 88% in optimum conditions, and the influences of initial MXC concentration, pH, and temperature on the degradation rate were evaluated. The metabolites including 2-methylpentane, 3-methylpentane, and n-pentane of MXC were identified, and possible degradation mechanisms were proposed. Overall, this work successfully demonstrates not only the ability to degrade MXC in different circumstances but also provides a new idea for environmental remediation in the future.

Keywords: Fe3O4@Fe(0); Immobilization; Laccase; Methoxychlor degradation.

MeSH terms

Catalysis
Enzymes, Immobilized*
Methoxychlor*
Soil
Wastewater

Substances

Methoxychlor
Enzymes, Immobilized
Wastewater
Soil