Microbial induced phosphate precipitation accelerate lead mineralization to alleviate nucleotide metabolism inhibition and alter Penicillium oxalicum's adaptive cellular machinery

Abstract

Microbial-induced phosphate (P) precipitation (MIPP) based on P-solubilizing microorganisms (PSM) is regarded as a promising approach to bioimmobilize environmental lead (Pb). Nevertheless, the underlying changes of Pb²⁺ biotoxicity in PSM during MIPP process were rarely discussed. The current study explored the Pb²⁺ immobilization and metabolic changes in PSM Penicillium oxalicum postexposure to Pb²⁺ and/or tricalcium phosphate (TCP). TCP addition significantly increased soluble P concentrations, accelerated extracellular Pb mineralization, and improved antioxidative enzyme activities in P. oxalicum during MIPP process. Secondary Pb²⁺ biomineralization products were measured as hydroxypyromorphite [Pb₁₀(PO₄)₆(OH)₂]. Using untargeted metabolomic and transcriptomics, we found that Pb²⁺ exposure stimulated the membrane integrity deterioration and nucleotide metabolism obstruction of P. oxalicum. Correspondingly, P. oxalicum could produce higher levels of gamma-aminobutyric acid (GABA) to enhance the adaptive cellular machineries under Pb²⁺ stress. While the MIPP process improved extracellular Pb²⁺ mineralization, consequently alleviating the nucleotide metabolism inhibition and membrane deterioration. Multi-omics results suggested that GABA degradation pathway was stimulated for arginine biosynthesis and TCA cycle after Pb²⁺ mineralization. These results provided new biomolecular information underlying the Pb²⁺ exposure biotoxicities to microorganisms in MIPP before the application of this approach in environmental Pb²⁺ remediation.

Keywords: Metabolomics; Microbial-induced phosphate precipitation; Pb bioimmobilization; Penicillium oxalicum; Transcriptomics.