A highly selective probe for ratiometric imaging peroxynitrite in living cells and in vivo

Jia-Qi Xu; Meng-Jiao Gao; Jin-Shuang Guo; Yi-Hua Wang; Ran Wei; Ya-Li Meng; Yan-Fei Kang

doi:10.1016/j.bioorg.2022.106055

A highly selective probe for ratiometric imaging peroxynitrite in living cells and in vivo

Bioorg Chem. 2022 Nov:128:106055. doi: 10.1016/j.bioorg.2022.106055. Epub 2022 Jul 22.

Authors

Jia-Qi Xu¹, Meng-Jiao Gao¹, Jin-Shuang Guo², Yi-Hua Wang³, Ran Wei¹, Ya-Li Meng⁴, Yan-Fei Kang⁵

Affiliations

¹ College of Laboratory Medicine, Hebei Key Laboratory of Quality & Safety Analysis-Testing for Agro-Products and Food, Zhang Jiakou Key Laboratory of Organic Light Functional Materials, Hebei North University, Zhangjiakou 075000, Hebei Province, PR China.
² Shandong Center for Food and Drug Evaluation and Inspection, Jinan, Shandong 250000, PR China; State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, PR China.
³ College of Chemical Engineering, Lanzhou University of Arts and Sciences, Lanzhou 730010, PR China.
⁴ College of Laboratory Medicine, Hebei Key Laboratory of Quality & Safety Analysis-Testing for Agro-Products and Food, Zhang Jiakou Key Laboratory of Organic Light Functional Materials, Hebei North University, Zhangjiakou 075000, Hebei Province, PR China. Electronic address: limengyajiajia@163.com.
⁵ College of Laboratory Medicine, Hebei Key Laboratory of Quality & Safety Analysis-Testing for Agro-Products and Food, Zhang Jiakou Key Laboratory of Organic Light Functional Materials, Hebei North University, Zhangjiakou 075000, Hebei Province, PR China. Electronic address: kangyanfei172@163.com.

PMID: 35905693
DOI: 10.1016/j.bioorg.2022.106055

Abstract

In this study, we constructed and displayed a ratiometric fluorescent probe JQ-2 for detecting ONOO^-. The probe JQ-2 showed a ratiometric signal for visualizing ONOO^- with a rapid response and high selectivity over a panel of biological analytes. Moreover, the JQ-2 has near-infrared emission (657 nm), which provides an excellent basis for the practical application in biological systems. The probe JQ-2 possessed low cytotoxicity and excellent cell membrane permeability, which can specifically visualize the exogenous and endogenous ONOO^- in vitro and vivo by emission in two channels. Meanwhile, JQ-2 can be used for diagnosing drug-induced liver injury by visualizing and monitoring the fluctuations of endogenous ONOO^-. Therefore, JQ-2 provided a potential tool for precisely detecting the fluctuation of ONOO^- in biological systems to understand physiological and pathological process.

Keywords: Bioimaging; Drug-induced liver injury; Fluorescence; Peroxide nitrite.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chemical and Drug Induced Liver Injury*
Fluorescent Dyes
Humans
Optical Imaging
Peroxynitrous Acid*

Substances

Fluorescent Dyes
Peroxynitrous Acid