Spectroscopy studies of interaction hypericin with an anti-cancer therapy drug doxorubicin

Monika Pietrzak; Mariusz Szabelski; Grzegorz Wołąkiewicz; Zbigniew Wieczorek

doi:10.1016/j.bpc.2022.106858

Spectroscopy studies of interaction hypericin with an anti-cancer therapy drug doxorubicin

Biophys Chem. 2022 Sep:288:106858. doi: 10.1016/j.bpc.2022.106858. Epub 2022 Jul 19.

Authors

Monika Pietrzak¹, Mariusz Szabelski², Grzegorz Wołąkiewicz², Zbigniew Wieczorek²

Affiliations

¹ Department of Physics and Biophysics, University of Warmia and Mazury in Olsztyn, Oczapowskiego 4, 10-719 Olsztyn, Poland. Electronic address: pietrzak@uwm.edu.pl.
² Department of Physics and Biophysics, University of Warmia and Mazury in Olsztyn, Oczapowskiego 4, 10-719 Olsztyn, Poland.

PMID: 35905651
DOI: 10.1016/j.bpc.2022.106858

Abstract

The presented study was designed to estimate the ability of hypericin to interact with the anticancer drug doxorubicin. The hetero-association of hypericin and doxorubicin was investigated with absorption and fluorescence spectroscopy methods in aqueous solution of DMSO in two-component mixtures: doxorubicin-hypericin and three component mixtures: DNA-doxorubicin-hypericin. The data indicate that hypericin forms complexes with doxorubicin and that the association constants are on the order of 300,000 M^-1 in a buffer with 30% DMSO content. The absorption spectra of the hypericin - doxorubicin complexes were examined as well. Owing to its ability to interact with flat aromatic compounds, hypericin may potentially be used as an interceptor molecule to detoxification of patients after chemotherapy.

Keywords: Absorption; Doxorubicin; Fluorescence; Hetero-association; Hypericin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anthracenes
Dimethyl Sulfoxide*
Doxorubicin / chemistry
Doxorubicin / pharmacology
Humans
Perylene* / analogs & derivatives
Perylene* / chemistry
Spectrometry, Fluorescence

Substances

Anthracenes
Perylene
hypericin
Doxorubicin
Dimethyl Sulfoxide