Antitumor Potential and Structure Characterization of Polysaccharides From Lagotis brevituba Maxim in the Tibetan Plateau

Ruixue Gong; Weiguo Cao; Haijun Huang; Bao Yu; Huan Chen; Wei Tao; Quji Luorong; Juan Luo; Dan Zhang

doi:10.3389/fnut.2022.921892

Antitumor Potential and Structure Characterization of Polysaccharides From Lagotis brevituba Maxim in the Tibetan Plateau

Front Nutr. 2022 Jul 12:9:921892. doi: 10.3389/fnut.2022.921892. eCollection 2022.

Authors

Ruixue Gong¹, Weiguo Cao¹, Haijun Huang¹, Bao Yu¹, Huan Chen¹, Wei Tao¹, Quji Luorong¹, Juan Luo¹, Dan Zhang¹

Affiliation

¹ College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing, China.

Abstract

This study purified two polysaccharides (LBMPs) from Lagotis brevituba Maxim in several steps. The chemical structure of LBMP-2 was determined by HPGPC, FT-IR, IC, ¹H and ¹³C NMR, AFM, SEM, and TEM. The results show that LBMP-2 was mainly composed of GalA, and the Mw of LBMP-2 is 23.799 kDa. In addition, the antioxidant activity, and the antitumor activity in vitro and in vivo were studied. LBMP-2 has excellent antioxidant and antitumor capacity. The inhibition of tumor cell proliferation in vitro may result in the inhibition of aerobic respiration and glycolysis. Tumor growth inhibition in vivo may inhibit the expression of AMPK in tumors and enhance spleen function. Compared with conventional chemotherapy drug cyclophosphamide, LBMP-2 is less harmful to the body and safer. Therefore, LBMP-2 provides a potential source of antitumor drugs.

Keywords: antioxidant; antitumor; isolation; purification; structure analysis.