Deoxynivalenol fluorescence aptasensor based on AuCu bimetallic nanoclusters and MoS2

Mikrochim Acta. 2022 Jul 28;189(8):296. doi: 10.1007/s00604-022-05385-4.

Abstract

Aptamers against deoxynivalenol (DON) were selected through capture-systematic evolution of ligands by exponential enrichment. Through isothermal titration calorimetry and fluorimetric assay, aptamer candidate DN-2 demonstrated good affinity to DON with Kd value of 40.36 ± 6.32 nM. Accordingly, a Forster resonance energy transfer aptasensor was fabricated by using the aptamer DN-2 combined with AuCu bimetallic nanoclusters as energy donor and MoS2 nanosheets as energy acceptor. Under the optimal conditions, the fluorescence response was utilized for DON quantitative determination ranging from 5 to 100 ng/mL with a detection limit of 1.87 ng/mL. The practical application of this method was verified in maize flour samples and demonstrated a satisfied recovery of 94.6 ~ 103.1%. The obtained aptamers and their application in DON determination provide a new tool for DON monitoring in various foodstuff.

Keywords: Aptamer; AuCu bimetallic nanoclusters; DON; FRET; MoS2 nanosheets.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide* / chemistry
  • Fluorescence Resonance Energy Transfer / methods
  • Fluorometry
  • Molybdenum / chemistry
  • Trichothecenes

Substances

  • Aptamers, Nucleotide
  • Trichothecenes
  • Molybdenum
  • deoxynivalenol