A Novel High-Throughput Phenotyping Hydroponic System for Nitrogen Deficiency Studies in Arabidopsis thaliana

Lucia M Acosta-Gamboa; Zachary C Campbell; Fei Gao; Benjamin Babst; Argelia Lorence

doi:10.1007/978-1-0716-2537-8_3

A Novel High-Throughput Phenotyping Hydroponic System for Nitrogen Deficiency Studies in Arabidopsis thaliana

Methods Mol Biol. 2022:2539:19-24. doi: 10.1007/978-1-0716-2537-8_3.

Authors

Lucia M Acosta-Gamboa¹, Zachary C Campbell¹, Fei Gao², Benjamin Babst³, Argelia Lorence^{4

5}

Affiliations

¹ Arkansas Biosciences Institute, Arkansas State University, Jonesboro, AR, USA.
² Arkansas Forest Resources Center, Division of Agriculture, University of Arkansas System, Monticello, AR, USA.
³ Arkansas Forest Resources Center, Division of Agriculture, University of Arkansas System, Monticello, AR, USA. babst@uamont.edu.
⁴ Arkansas Biosciences Institute, Arkansas State University, Jonesboro, AR, USA. alorence@astate.edu.
⁵ Department of Chemistry and Physics, Arkansas State University, Jonesboro, AR, USA. alorence@astate.edu.

PMID: 35895192
DOI: 10.1007/978-1-0716-2537-8_3

Abstract

High-throughput phenotyping enables the temporal detection of subtle changes in plant plasticity and adaptation to different conditions, such as nitrogen deficiency, in an accurate, nondestructive, and unbiased way. Here, we describe a protocol to assess the contribution of nitrogen addition or deprival using an image-based system to analyze plant phenotype. Thousands of images can be captured throughout the life cycle of Arabidopsis, and those images can be used to quantify parameters such as plant growth (area, caliper length, diameter, etc.), in planta chlorophyll fluorescence, and in planta relative water content.

Keywords: Arabidopsis; High-throughput phenotyping; Hydroponics; Nitrogen.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Arabidopsis* / genetics
Hydroponics
Nitrogen
Phenotype
Plant Development
Plants

Substances

Nitrogen