Recombinant Muscovy Duck Parvovirus Led to Ileac Damage in Muscovy Ducklings

Jiahui He; Yukun Zhang; Zezhong Hu; Luxuan Zhang; Guanming Shao; Zi Xie; Yu Nie; Wenxue Li; Yajuan Li; Liyi Chen; Benli Huang; Fengsheng Chu; Keyu Feng; Wencheng Lin; Hongxin Li; Weiguo Chen; Xinheng Zhang; Qingmei Xie

doi:10.3390/v14071471

Recombinant Muscovy Duck Parvovirus Led to Ileac Damage in Muscovy Ducklings

Viruses. 2022 Jul 3;14(7):1471. doi: 10.3390/v14071471.

Authors

Jiahui He^{1

2

3}, Yukun Zhang^{1

2

3}, Zezhong Hu^{1

2

3}, Luxuan Zhang⁴, Guanming Shao^{1

2

3}, Zi Xie^{1

2

3}, Yu Nie^{1

2

3}, Wenxue Li^{1

2

3}, Yajuan Li^{1

2

3}, Liyi Chen^{1

2

3}, Benli Huang^{1

2

3}, Fengsheng Chu^{1

2

3}, Keyu Feng^{1

2

3

5}, Wencheng Lin^{1

2

3

5}, Hongxin Li^{1

2

3

5}, Weiguo Chen^{1

2

3

5}, Xinheng Zhang^{1

2

3

5}, Qingmei Xie^{1

2

3

5}

Affiliations

¹ Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
² Guangdong Engineering Research Center for Vector Vaccine of Animal Virus, Guangzhou 510642, China.
³ South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, China.
⁴ School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou 510006, China.
⁵ Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, China.

Abstract

Waterfowl parvovirus (WPFs) has multiple effects on the intestinal tract, but the effects of recombinant Muscovy duck parvovirus (rMDPV) have not been elucidated. In this study, 48 one-day-old Muscovy ducklings were divided into an infected group and a control group. Plasma and ileal samples were collected from both groups at 2, 4, 6, and 8 days post-infection (dpi), both six ducklings at a time. Next, we analyzed the genomic sequence of the rMDPV strain. Results showed that the ileal villus structure was destroyed seriously at 4, 6, 8 dpi, and the expression of ZO-1, Occludin, and Claudin-1 decreased at 4, 6 dpi; 4, 6, 8 dpi; and 2, 6 dpi, respectively. Intestinal cytokines IFN-α, IL-1β and IL-6 increased at 6 dpi; 8 dpi; and 6, 8 dpi, respectively, whereas IL-2 decreased at 6, 8 dpi. The diversity of ileal flora increased significantly at 4 dpi and decreased at 8 dpi. The bacteria Ochrobactrum and Enterococcus increased and decreased at 4, 8 dpi; 2, 4 dpi, respectively. Plasma MDA increased at 2 dpi, SOD, CAT, and T-AOC decreased at 2, 4, 8 dpi; 4, 8 dpi; and 4, 6, 8 dpi, respectively. These results suggest that rMDPV infection led to early intestinal barrier dysfunction, inflammation, ileac microbiota disruption, and oxidative stress.

Keywords: ileac microflora; inflammation; molecular characterization; oxidative stress; waterfowl parvovirus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Ducks
Parvoviridae Infections* / veterinary
Parvovirinae* / genetics
Parvovirus* / genetics
Poultry Diseases*

Supplementary concepts

Avian dependoparvovirus 1

Grants and funding

This research was funded by the Guangdong Basic and Applied Basic Research Foundation (2019B1515210034, 2019A1515012006), the Key Research and Development Program of Guangdong Province (2020B020222001), the Construction of Modern Agricultural Science and Technology Innovation Alliance in Guangdong Province (2020KJ128), the National Natural Science Foundation of China (31902252), the Special Project of National Modern Agricultural Industrial Technology System (CARS-41), the National Modern Agricultural Industry Science and Technology Innovation Center in Guangzhou (2018kczx01), the National Key R&D Program of China (2017YFD0502001), the Creation of a Triple Chimeric Vaccine (rIBV-ND-H9) Using Avian Infectious Bronchitis Attenuated D90 as a Vector (2017KZDM008), and the Guangzhou Science and Technology Bureau (GZKTP201930).