Effect of Pre-Induced Mesenchymal Stem Cell-Coated Cellulose/Collagen Nanofibrous Nerve Conduit on Regeneration of Transected Facial Nerve

GwangWon Cho; Changjong Moon; Nagarajan Maharajan; Mary Jasmin Ang; Minseong Kim; Chul Ho Jang

doi:10.3390/ijms23147638

Effect of Pre-Induced Mesenchymal Stem Cell-Coated Cellulose/Collagen Nanofibrous Nerve Conduit on Regeneration of Transected Facial Nerve

Int J Mol Sci. 2022 Jul 11;23(14):7638. doi: 10.3390/ijms23147638.

Authors

GwangWon Cho^{1

2}, Changjong Moon³, Nagarajan Maharajan^{1

2}, Mary Jasmin Ang⁴, Minseong Kim⁵, Chul Ho Jang⁶

Affiliations

¹ Department of Biology, College of Natural Science, Chosun University, Gwangju 61452, Korea.
² Department of Life Science, BK21-Plus Research Team for Bioactive Control Technology, Chosun University, Gwangju 61452, Korea.
³ Department of Veterinary Anatomy, College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Gwangju 61186, Korea.
⁴ College of Veterinary Medicine, University of the Philippines Los Baños, Los Baños 4031, Philippines.
⁵ Advanced Biomaterial Team, Medical Device Development Center, Daegu-Gyeongbuk Medical Innovation Foundation, Dong-gu 41061, Korea.
⁶ Department of Otolaryngology, Chonnam National University Medical School, Gwangju 61469, Korea.

Abstract

(1) Objective: In order to evaluate the effect of a pre-induced mesenchymal stem cell (MSC)-coated cellulose/collagen nanofibrous nerve conduit on facial nerve regeneration in a rat model both in vitro and in vivo. (2) Methods: After fabrication of the cellulose/collagen nanofibrous conduit, its lumen was coated with either MSCs or pre-induced MSCs. The nerve conduit was then applied to the defective main trunk of the facial nerve. Rats were randomly divided into three treatment groups (n = 10 in each): cellulose/collagen nanofiber (control group), cellulose/collagen nanofiber/MSCs (group I), and cellulose/collagen nanofiber/pre-induced MSCs (group II). (3) Results Fibrillation of the vibrissae of each group was observed, and action potential threshold was compared 8 weeks post-surgery. Histopathological changes were also observed. Groups I and II showed better recovery of vibrissa fibrillation than the control group. (4) Conclusions: Group II, treated with the pre-induced MSC-coated cellulose/collagen nanofibrous nerve conduit, showed the highest degree of recovery based on functional and histological evaluations.

Keywords: cellulose; collagen; nanofibrous nerve conduit; nerve regeneration; pre-induced mesenchymal stem cell.

Publication types

Evaluation Study

MeSH terms

Animals
Cellulose* / pharmacology
Coated Materials, Biocompatible
Collagen* / pharmacology
Disease Models, Animal
Facial Nerve* / drug effects
Facial Nerve* / physiology
Guided Tissue Regeneration
Mesenchymal Stem Cells* / physiology
Nanofibers* / administration & dosage
Nerve Regeneration* / drug effects
Nerve Regeneration* / physiology
Rats
Sciatic Nerve / pathology
Tissue Scaffolds

Substances

Coated Materials, Biocompatible
Cellulose
Collagen

Grants and funding

This study was supported by the National Research Foundation of Korea (NRF) grant from the Korean government (MSIT) (No. 2021R1A2C101417611) and a grant (BCRI 20-032) from Chonnam National University Hospital Biomedical Research Institute.