Association of TNF-α rs1800629 with Adult Acute B-Cell Lymphoblastic Leukemia

Ezeldine K Abdalhabib; Abdulrahman Algarni; Muhammad Saboor; Fehaid Alanazi; Ibrahim K Ibrahim; Ayman H Alfeel; Abdullah M Alanazi; Abdulmajeed M Alanazi; Abdulaziz M Alruwaili; Muath H Alanazi; Nahla A Alshaikh

doi:10.3390/genes13071237

Association of TNF-α rs1800629 with Adult Acute B-Cell Lymphoblastic Leukemia

Genes (Basel). 2022 Jul 13;13(7):1237. doi: 10.3390/genes13071237.

Affiliations

¹ Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakakah P.O. Box 42421, Saudi Arabia.
² Department of Medical Laboratory Technology, College of Applied Medical Sciences, Northern Border University, Arar P.O. Box 91431, Saudi Arabia.
³ Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Jazan University, Jazan P.O. Box 45142, Saudi Arabia.
⁴ Medical Research Center, Jazan University, Jazan P.O. Box 45142, Saudi Arabia.
⁵ Department of Hematology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum P.O. Box 12702, Sudan.
⁶ Department of Medical Laboratory Sciences, College of Health Sciences, Gulf Medical University, Ajman P.O. Box 4184, United Arab Emirates.
⁷ Gurayyat Health Affair, Regional Laboratory and Central Blood Bank, Ministry of Health, Gurayyat P.O. Box 77413, Saudi Arabia.

Abstract

TNF−α influences lymphomagenesis by upregulating proinflammatory and antiapoptotic pathways. In this study, we evaluated the frequency of TNF−α rs1800629 (−308 G>A) polymorphism in newly diagnosed adult patients with acute lymphoblastic leukemia (ALL) and its correlation with age at diagnosis, gender and subtype of ALL. In this case control study, a total of 330 individuals were recruited, including 165 newly diagnosed adult patients with ALL, from the Radiation and Isotope Center in Khartoum (RICK) and 165 healthy normal controls. TNF−α rs1800629 polymorphism was tested through allele-specific polymerase chain reaction (PCR) assay. The frequency of the rs1800629 GA genotype was high (70.9% vs. 60%, OR = 1.84) in the patient group as compared to healthy controls, whereas GG and AA genotypes did not exhibit any statistically significant difference between controls and patients. Based on subtype, GG and GA rs1800629 genotypes showed increased risk of B-ALL (OR 0.46 and 2.12, respectively), whereas rs1800629 GG, GA and AA genotypes did not show any disease association with T-ALL (p > 0.05). Age at diagnosis and gender did not exhibit any association of rs1800629 with ALL in the patient group. In conclusion, rs1800629 is associated with high risk of adult B-ALL, with an insignificant effect of age at diagnosis and gender.

Keywords: TNF–α; acute lymphoblastic leukemia; polymorphism; rs1800629.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Case-Control Studies
Genetic Predisposition to Disease
Humans
Polymorphism, Single Nucleotide
Precursor Cell Lymphoblastic Leukemia-Lymphoma* / genetics
Tumor Necrosis Factor-alpha / genetics*

Substances

TNF protein, human
Tumor Necrosis Factor-alpha

Grants and funding

The authors extend their appreciation to the Deputyship for Research & Innovation, Ministry of Education, Saudi Arabia, for funding this research work through project number “IF_2020_NBU_222”.