Developments of Conventional and Microfluidic Flow Cytometry Enabling High-Throughput Characterization of Single Cells

Minruihong Wang; Hongyan Liang; Xiao Chen; Deyong Chen; Junbo Wang; Yuan Zhang; Jian Chen

doi:10.3390/bios12070443

Developments of Conventional and Microfluidic Flow Cytometry Enabling High-Throughput Characterization of Single Cells

Biosensors (Basel). 2022 Jun 23;12(7):443. doi: 10.3390/bios12070443.

Authors

Minruihong Wang^{1

2}, Hongyan Liang^{1

3}, Xiao Chen^{1

2}, Deyong Chen^{1

2

3}, Junbo Wang^{1

2

3}, Yuan Zhang⁴, Jian Chen^{1

2

3}

Affiliations

¹ State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China.
² School of Future Technology, University of Chinese Academy of Sciences, Beijing 100049, China.
³ School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100049, China.
⁴ Materials Genome Institute, Shanghai University, Shanghai 200444, China.

Abstract

This article first reviews scientific meanings of single-cell analysis by highlighting two key scientific problems: landscape reconstruction of cellular identities during dynamic immune processes and mechanisms of tumor origin and evolution. Secondly, the article reviews clinical demands of single-cell analysis, which are complete blood counting enabled by optoelectronic flow cytometry and diagnosis of hematologic malignancies enabled by multicolor fluorescent flow cytometry. Then, this article focuses on the developments of optoelectronic flow cytometry for the complete blood counting by comparing conventional counterparts of hematology analyzers (e.g., DxH 900 of Beckman Coulter, XN-1000 of Sysmex, ADVIA 2120i of Siemens, and CELL-DYN Ruby of Abbott) and microfluidic counterparts (e.g., microfluidic impedance and imaging flow cytometry). Future directions of optoelectronic flow cytometry are indicated where intrinsic rather than dependent biophysical parameters of blood cells must be measured, and they can replace blood smears as the gold standard of blood analysis in the near future.

Keywords: hematology analyzer; microfluidic impedance and imaging flow cytometry; optoelectronic flow cytometry; single-cell analysis.

Publication types

Review

MeSH terms

Blood Cell Count
Flow Cytometry
Hematologic Tests* / methods
Microfluidics*
Single-Cell Analysis

Abstract

Publication types

MeSH terms

Grants and funding