Large-Scale Profiling on lncRNAs in Human Platelets: Correlation with Platelet Reactivity

Yeying Sun; Rongrong Liu; Xiangwen Xia; Luchuan Xing; Jing Jiang; Weihua Bian; Wendy Zhang; Chunhua Wang; Chunxiang Zhang

doi:10.3390/cells11142256

Large-Scale Profiling on lncRNAs in Human Platelets: Correlation with Platelet Reactivity

Cells. 2022 Jul 21;11(14):2256. doi: 10.3390/cells11142256.

Authors

Yeying Sun¹, Rongrong Liu¹, Xiangwen Xia¹, Luchuan Xing¹, Jing Jiang¹, Weihua Bian^{1

2}, Wendy Zhang², Chunhua Wang¹, Chunxiang Zhang³

Affiliations

¹ US-China Institute for Translational Medicine, College of Pharmacy, Binzhou Medical University, Yantai 264000, China.
² Department of Biomedical Engineering, School of Medicine, The University of Alabama at Birmingham, Birmingham, AL 35233, USA.
³ Department of Cardiology, The Affiliated Hospital of Southwest Medical University, Key Laboratory of Medical Electrophysiology of Ministry of Education, Institute of Cardiovascular Research, Nucleic Acid Medicine of Luzhou Key Laboratory, Metabolic Vascular Disease Key Laboratory of Sichuan Province, Southwest Medical University, Luzhou 646000, China.

Abstract

Recently, long noncoding RNAs (lncRNAs) have been key regulators for both mRNAs and proteins in nucleated cells. However, the expression profiles of lncRNAs in non-nucleated cells such as platelets are currently unclear. In this study, we determined the expression profiles of lncRNAs in human platelets. We found that 6109 lncRNAs were expressed in human platelets. Interestingly, 338 lncRNAs were differentially expressed in hyperreactive and hyporeactive platelets. Bioinformatics' analysis revealed that these aberrantly expressed lncRNAs might be related to platelet activity and other platelet functions. To provide a proof of concept, we measured the expression levels of PARLncRNA-1, a down-regulated lncRNA of hyperreactive platelets, in platelets from 12 patients with acute myocardial infarction and their controls. We found that the lncRNA was also significantly down-regulated in platelets from patients, which was partially reversed by treatment with aspirin a known antiplatelet drug. LncRNAs may represent a novel class of modulators for platelet functions.

Keywords: hyperreactive; hyporeactive; long noncoding RNAs; platelet function; platelets.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Profiling
Humans
RNA, Long Noncoding* / genetics
RNA, Long Noncoding* / metabolism
RNA, Messenger / genetics

Substances

RNA, Long Noncoding
RNA, Messenger

Grants and funding

This work was supported by the National Natural Science Foundation of China (Grant No. 82030007, 81670398 and 91639102) and the funding of Taishan Scholars of Shandong Province to Binzhou Medical University. This work was also supported in part by the Key Project of Zhejiang Provincial Natural Science Foundation (LZ17H250001) and Sichuan Science and Technology Program (2022YFS0578).