Kinetic Changes in B7 Costimulatory Molecules and IRF4 Expression in Human Dendritic Cells during LPS Exposure

Biomolecules. 2022 Jul 8;12(7):955. doi: 10.3390/biom12070955.

Abstract

A key aspect of the inflammatory phenomenon is the involvement of costimulatory molecules expressed by antigen-presenting cells (APCs) and their ability to secrete cytokines to set instructions for an adaptive immune response and to generate tolerance or inflammation. In a novel integrative approach, we aimed to evaluate the kinetic expression of the membrane and soluble B7 costimulatory molecules CD86, ICOS-L, PDL1, PDL2, the transcription factor Interferon Regulatory Factor 4 (IRF4), and the cytokines produced by monocyte-derived dendritic cells (Mo-DCs) after challenging them with different concentrations of stimulation with E. coli lipopolysaccharide (LPS) for different lengths of time. Our results showed that the stimuli concentration and time of exposure to an antigen are key factors in modulating the dynamic expression pattern of membrane and soluble B7 molecules and cytokines.

Keywords: B7; CD86; ICOS-L; IRF4; LPS; PDL1; PDL2; costimulatory molecules; cytokines; dendritic cells; soluble costimulatory molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • B7 Antigens / metabolism
  • B7-1 Antigen* / metabolism
  • Cytokines / metabolism
  • Dendritic Cells
  • Escherichia coli / metabolism
  • Humans
  • Interferon Regulatory Factors / genetics
  • Interferon Regulatory Factors / metabolism
  • Lipopolysaccharides* / metabolism
  • Lipopolysaccharides* / pharmacology

Substances

  • B7 Antigens
  • B7-1 Antigen
  • Cytokines
  • Interferon Regulatory Factors
  • Lipopolysaccharides

Grants and funding

This work was supported by the Institute of Ophthalmology, “Fundación Conde de Valenciana,” and “Laboratorio Nacional para Servicios Especializados de Investigación, Desarrollo e Innovación (I + D + i) para Farmoquímicos y Biotecnológicos, LANSEIDI-FarBiotec-CONACyT.” Velazquez-Soto, H. received fellowship 294674 from CONACyT during his doctoral studies in Programa de Doctorado en Ciencias Médicas, Odontológicas y de la Salud (Farmacología Clínica), Universidad Nacional Autónoma de México (UNAM). Real-San-Miguel, F., with CVU 917729, received a fellowship from CONACyT during her masters studies in Programa de Maestría en Ciencias Médicas, Odontológicas y de la Salud (Farmacología Clínica), Universidad Nacional Autónoma de México (UNAM).