Biocompatible silane adhesion layer on titanium implants improves angiogenesis and osteogenesis

Yuyu Zhao; Yonghua Sun; Ruiyue Hang; Runhua Yao; Yi Zhang; Di Huang; Xiaohong Yao; Long Bai; Ruiqiang Hang

doi:10.1016/j.bioadv.2022.213033

Biocompatible silane adhesion layer on titanium implants improves angiogenesis and osteogenesis

Biomater Adv. 2022 Aug:139:213033. doi: 10.1016/j.bioadv.2022.213033. Epub 2022 Jul 16.

Authors

Yuyu Zhao¹, Yonghua Sun¹, Ruiyue Hang¹, Runhua Yao¹, Yi Zhang¹, Di Huang², Xiaohong Yao³, Long Bai⁴, Ruiqiang Hang⁵

Affiliations

¹ Shanxi Key Laboratory of Biomedical Metal Materials, College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
² Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China; Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan 030032, China.
³ Shanxi Key Laboratory of Biomedical Metal Materials, College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China. Electronic address: xhyao@tyut.edu.cn.
⁴ Institute of Translational Medicine, Shanghai University, Shanghai, 200444 China; Engineering Research Center for Biomedical Materials of Ministry of Education, College of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address: bailong@ecust.edu.cn.
⁵ Shanxi Key Laboratory of Biomedical Metal Materials, College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China. Electronic address: hangruiqiang@tyut.edu.cn.

PMID: 35882124
DOI: 10.1016/j.bioadv.2022.213033

Abstract

Silane adhesion layer strategy has been widely used to covalently graft biomolecules to the titanium implant surface, thereby conferring the implant bioactivity to ameliorate osseointegration. However, few researchers pay attention to the effects of silanization parameters on biocompatibility and biofunctionality of the silane adhesion layers. Accordingly, the present study successfully fabricated the silane adhesion layers with different thickness, intactness, and surface morphologies by introducing 3-aminopropyltriethoxysilane on the alkali-treated titanium surface in time-varied processing of silanization. The regulatory effects of the silane adhesion layers on angiogenesis and osteogenesis were assessed in vitro. Results showed that the prolonged silanization processing time increased the thickness and intactness of the silane adhesion layer and significantly improved its biocompatibility. Notably, the silane adhesion layer prepared after 12 h of silanization exhibited a brain-like surface morphology and benefited the adhesion and proliferation of endothelial cells (ECs) and osteoblasts (OBs). Moreover, the layer promoted angiogenesis via stimulating vascular endothelial growth factor (VEGF) secretion and nitric oxide (NO) production of ECs. Simultaneously, it improved osteogenesis by enhancing alkaline phosphatase (ALP) activity, collagen secretion, and extracellular matrix mineralization of OBs. This work systematically investigated the biocompatibility and biofunctionality of the modified silane adhesion layers, thus providing valuable references for their application in covalently grafting biomolecules on the titanium implant surface.

Keywords: Adhesion layer; Angiogenesis; Osteogenesis; Silanization; Titanium.

MeSH terms

Endothelial Cells
Osteogenesis*
Silanes / pharmacology
Surface Properties
Titanium* / pharmacology
Vascular Endothelial Growth Factor A / pharmacology

Substances

Silanes
Vascular Endothelial Growth Factor A
Titanium